...foot shock that has become associated with a visual or auditory cue. The hippocampus has long been known...every other advanced technique in neuroscience it has a bit of a steep learning curve and many opportunities...within your own neuroscience sub-discipline that has published using the technique and ask if they would...sophistication of mouse transgenics. Everything that has been described is intended for the mouse; however... behavioral models. Beyond rodents, optogenetics has even been applied to other species such as non-human...

...marketing campaign! MIT Green Labs has a lot of resources about this, and even has a list of biological samples...are stored long term at -70 °C. My Green Lab also has resources and even a freezer challenge that your ...

... backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold science-themed...fan of Addgene or our very cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed...background.           Maybe your viral research has taken you in the direction of COVID-19. We have a...

...by Neville Sanjana. This fantastic research tool has been requested from the repository nearly 1,000 times...provided by the Zhang lab. Even though lentiCRISPR v2 has only been available for a little over a year and ...and a half, a small sampling of the research it has already been used for includes work probing mTOR signaling...

...need new epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations...effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not reduce...observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects on cell size...

...avoid selecting for cells in which the construct has randomly integrated into the genome, a negative selection... opposed to screen for cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance...still functions normally because one of its exons has simply been replaced with the same sequence flanked...flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure 4: ...second the floxed exon.   After recombination has occurred, you'll first remove the resistance marker...

... in NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids...we analyze as part of our quality control process has steadily grown. On a busy week, we may need to analyze...our quality control process. Consequently our team has refined our use of the BLAST web browser interface...also enjoy seeing how our quality control process has changed with the introduction of next generation ...BLAST (blastp), and Translated BLAST (blastx). NCBI has a terrific getting-started guide for BLAST, which...

... makes software good? Fortunately, that question has been thoroughly answered by experts in human-computer...specialized controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. ...protein sequence match even when the DNA sequence has changed. Identifying plasmid control elements Coding... with these extensive efforts, such an algorithm has limitations. For instance, it could miss a common...

...Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize...various flux imbalances. The conventional approach has typically been to either systematically delete/overexpress...introduce heterologous genes, but the trend of research has shifted in recent years toward tinkering with regulatory.... Since the technique relies on base pairing, it has promise to be host-independent, beyond the oft published...

... favorite! Our mascot who has supported us over the past eight years now has a new friend to join them...

...agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a longer shelf life... It doesn’t degrade during long-term storage and has both high binding capacity and high protein retention... specialty low-background PVDF membrane, it also has higher background noise than nitrocellulose membranes... other hand, doesn’t require any pre-wetting and has lower background than PVDF. It is a little quicker...quicker to use, with high binding capacity, though it has lower retention after binding and washes. It’s less...and need to be read fairly soon after the reaction has started. Types of chemiluminescence enzymes The two...darkroom and a developer. Film is highly sensitive but has a limited dynamic range, which means it may take ...

...Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base editor from an evolved...in diverse bacteria from various environments. It has the advantage of stable genomic integration and is...Cre or Flp recombinases used to excise markers. It has the advantage that CRISPR efficiency can be controlled...

...studied but it is only the tip of the iceberg as it has been predicted that many others are still unknown...enzymes. In contrast to other Cas enzymes, Cas14 has not been found in bacterial genomes but only in the...add-on to a diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 ...

...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...reduced impact of scientists and science. The pandemic has demonstrated that, more than ever, we need all of...at University of Massachusetts Medical School. It has been supported by a Steering Committee and working...

...– to specific sequences of DNA has become commonplace. This ability has opened up a world of possibilities...the original paper says). In their hands, BsaI-HF has produced less reliable results. She also notes that...

...specific field(s). Most anyone who has spent any time in academia has likely had the opportunity to attend... one that no one sees a purpose in attending and has lost both its focus and critical mass of participants...their own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain...rebooted or disbanded all together once their purpose has been served. I hope that with some of the information...

...Biotech in May 2016 (1), NgAgo has received much attention and the plasmid has been requested by labs around...THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED ...spotlight and take a moment to remember that NgAgo has only been available for genome editing for a few ... synthesis, which could be less desirable if one has to target multiple sites in a single experiment. ...

...popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times; red, more than 50...available through Addgene. Publication: If the plasmid has been published, the paper in which it was first used... their new plasmid.  The Addgene Vector Database has more information about common vector backbones.  ...plasmid number here. Gene/insert: This describes what has been inserted into the backbone, and provides information...gene or DNA element of interest. If the depositor has indicated that there is a particular mutation found...

...Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab has switched over to..., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations,...isolated are those where the allelic exchange vector has undergone single crossover and integrated into the...to pTOX. However, we feel it has clear advantages, and are hopeful that continued development of allelic...the lab of Matthew Waldor at Harvard. Now that he has an efficient allelic exchange system, he is so excited...

...more commonly known: the fruit fly. The fruit fly has been used in scientific research for over a century... a transgenic mouse line. Over the years the fly has become an ideal model organism to study an array ...development, genetics, and the nervous system. The fly has also emerged as an important human disease model ... described in 1993 by Norbert Perrimon’s lab and has been continuously improved upon since then. If you...Zebrafish (Danio rerio) Since the 1960s, the zebrafish has gradually become an important model organism. They...Caenorhabditis elegans) The worm, Caenorhabditis elegans, has been used extensively as a model organism since the...