We narrowed to 395 results for: ICL
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TypeBlog Post...provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9 and/or gRNA... AAV transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/...
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Which Fluorescent Protein Should I Use?
TypeBlog Post...cytosol, which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. ... Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et.... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable skills...skills in that big job interview, check out this article from The Muse. Kai Strickland is a former Customer... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current...vials causes cavitation, protein aggregation, and particle formation. J Pharm Sci. 2015 Feb;104(2):602-11... -
An Introduction to Adenovirus
TypeBlog Post...including the E1 region). The space in the viral particle’s capacity that is made available by this deletion... et al., 1999). The ability of the adenovirus particle to activate the immune system, and of the infection... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...search tool lets you access plasmids from journal articles. From paper to plasmid Authors regularly indicate...also find the associated plasmids through the article’s PubMed ID (PMID) ...or DOI Or get some keywords... -
Lentiviral Vector Uses and Overview
TypeBlog Post...cells released transgene-containing lentiviral particles into the media, which could be collected for experimental...Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor ...activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver Cre-dependent... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the Drosophila...embryo. In flies with non-functional hedgehog, the denticles actually clump together forming one patch on the... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...this way data is moved from a static and isolated vehicle and becomes part of the dynamic and connected record...repositories have been launched and are now a main vehicle for making research data available for public access... -
Hot Plasmids - August 2020
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...cell will be transduced by no more than one viral particle, giving a low multiplicity of infection (MOI)....chances of a cell being infected by only one viral particle, but it also increases the chances that a cell... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...machinery to manufacture infectious viral particles. Those particles are collected to deliver the gene to ... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post... resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR...CRISPR methods for bacteria Find all articles about microbiology Resources on Addgene.org: Visit the CRISPR... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post... that compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted ...11. Jokitalo, Eija, et al. "Golgi clusters and vesicles mediate mitotic inheritance independently of the... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...are required for the production of lentiviral particles. The envelope plasmid typically encodes the vesicular...methods for titering IDLVs include detecting vector particle components or performing quantitative PCR titration... -
10 Steps to a Perfect Science Talk
TypeBlog Post...just be a career opportunity. The focus of this article will be talks for non-academic settings. Many of...talk for an academic position, see this very good article at NatureJobs: Interviews: The All-Important Job... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...is probably in the thousands! The goal of this article is to provide enough background for you to distinguish...Mammalian Vectors Looking for More Plasmids 101 Articles? Find them all at our Plasmids 101 Topic Page... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...then, is this: It’s the optimal number of viral particles per volume required to express the gene of interest...spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434-019-0075-6 Issa...