Addgene

We narrowed to 295 results for: IRES

Showing: 181 - 200 of 295 results

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15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...any genetic tool delivered by the AAV. AAVrg requires high titers for efficient retrograde transport...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...
How Addgene Creates Content

Type

Blog Post

... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...
Antibodies 101: Isotypes

Type

Blog Post

...deleted. Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...
From Lab Bench to Leadership: Transitioning into Management as a PhD

Type

Blog Post

... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace...
Hot Plasmids - February 2022

Type

Blog Post

...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids...
Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

...overlapping methylation Not affected Not affected DpnI Requires methylation for activity Not affected Not affected...
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Type

Blog Post

...in the 10-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...
Plasmids 101: Yeast Vectors

Type

Blog Post

... across many different cell types and each one requires different elements for vector propagation. This...
Visualizing Protein Turnover In Situ

Type

Blog Post

... of radioactive materials is highly regulated, requires designated work areas and come with health risks...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...
Plasmids 101: The protein expression toolbox

Type

Blog Post

...control all the time If your experimental system requires tunable control - or maybe you’re just a control...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

... example, the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially...
Developing Lab Management Software for Biology

Type

Blog Post

... itself. If something happens to the worm that requires a change of tube, there’s no clean way to separate...
Gendered Innovations: Why Does Sex of the Cell Matter?

Type

Blog Post

...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Type

Blog Post

...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

...Research at universities typically requires funding from a variety of government and academic institutions...

Showing: 181 - 200 of 295 results

Results per page:

15 Years of Addgene: The Top 15 Plasmids

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Viral Vectors 101: The AAV Retrograde Serotype

Viral Vectors 101: Viruses as Biological Tools

How Addgene Creates Content

Antibodies 101: Isotypes

From Lab Bench to Leadership: Transitioning into Management as a PhD

Hot Plasmids - February 2022

Plasmids 101: Methylation and Restriction Enzymes

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Plasmids 101: Yeast Vectors

Visualizing Protein Turnover In Situ

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Plasmids 101: The protein expression toolbox

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Developing Lab Management Software for Biology

Gendered Innovations: Why Does Sex of the Cell Matter?

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

5 Steps to Writing Grant Proposals for Grad Students

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