We narrowed to 298 results for: IRES

1. Plasmids 101: Inducible Promoters

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   Blog Post

   ...negative inducible pLac promoter. This promoter requires removal of the lac repressor (lacI protein) for...

2. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact nature...

3. A Deep Dive into BioBuilder

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   Blog Post

   ...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...

4. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...

5. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...

7. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   ...introduced directly into the cell. Microinjection requires the use of a fine needle to deliver nucleic acids...

8. How Addgene Creates Content

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   Blog Post

   ... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...

9. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...overlapping methylation Not affected Not affected DpnI Requires methylation for activity Not affected Not affected...

10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...in the 10-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which...

11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

12. Plasmids 101: Yeast Vectors

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    Blog Post

    ... across many different cell types and each one requires different elements for vector propagation. This...

13. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...control all the time If your experimental system requires tunable control - or maybe you’re just a control...

14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ... example, the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially...

15. Visualizing Protein Turnover In Situ

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    Blog Post

    ... of radioactive materials is highly regulated, requires designated work areas and come with health risks...

16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...

17. Developing Lab Management Software for Biology

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    Blog Post

    ... itself. If something happens to the worm that requires a change of tube, there’s no clean way to separate...

18. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What...

20. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ...Research at universities typically requires funding from a variety of government and academic institutions...