We narrowed to 298 results for: IRES

1. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   ... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   ...introduced directly into the cell. Microinjection requires the use of a fine needle to deliver nucleic acids...

4. A Deep Dive into BioBuilder

   Type

   Blog Post

   ...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...

5. How Addgene Creates Content

   Type

   Blog Post

   ... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...

6. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...

7. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact nature...

8. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   ...overlapping methylation Not affected Not affected DpnI Requires methylation for activity Not affected Not affected...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...in the 10-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

11. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ... across many different cell types and each one requires different elements for vector propagation. This...

12. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ...control all the time If your experimental system requires tunable control - or maybe you’re just a control...

13. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    ... of radioactive materials is highly regulated, requires designated work areas and come with health risks...

14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ... example, the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially...

15. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...

16. Developing Lab Management Software for Biology

    Type

    Blog Post

    ... itself. If something happens to the worm that requires a change of tube, there’s no clean way to separate...

17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    ...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...

18. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What...

19. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ...Research at universities typically requires funding from a variety of government and academic institutions...

20. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For example...