We narrowed to 5 results for: IRES

1. Lab Safety for Biosafety Levels One and Two

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   Protocol

   ...times when you may be working with a protocol that requires shaking or mixing, which may produce aerosols ... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves...

2. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...to the template DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine... thymine (T)] in an equal molar ratio. It also requires two unique single stranded DNA oligonucleotide...

3. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

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   Protocol

   ... ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction volume usually...

4. Colony Formation Titering Assay

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   Protocol

   ...cell lines and selection markers. Note this assay requires staining resistant colonies with crystal violet...

5. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use a "Universal...