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...cells). pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit...fusions that are on your insert. Typically you would list any tag or fusion protein in the order they appear... tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If there was also...fused to the C-terminal of your insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other...included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide change...proper way to denote an amino acid mutation is to list the one letter abbreviation of the wild type amino...

...Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas spp.,...stability, which influence the plasmid’s ability to establish and maintain itself within host cells.  The plasmid...bacteria in lab manipulations. Table 1 has a detailed list of the bacterial species these plasmids are compatible...compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and the bacterial species...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp., Rhizobium...campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...or another. Supported organisms: This section lists the genomes supported by each tool. The genomes ...agnostic and have currently available organisms listed in this section. CRISPR software advice for specific...

...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback...credit for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques...time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...

...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips...the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a deadline...everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the search ...spreadsheets for reagent storage locations, such as the list used to organize your cryo freezer. No more issues...

...Jessica Polka from Harvard Medical School and a long list of supporters from all of the research centers in...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...training excellence to begin effecting change.  Panelists call for action Each of the speakers in the panel...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...too; both with your research and with getting published.” Connie Cepko 1. Work hard. There will be periods...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

...magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your ...stories — whether I’m working with a talented journalist to shine light on living as a scientist in Beijing...research struggles in papers. They recount accomplishments in talks and interviews. They even tell short...tell good stories, they engage their readers or listeners thus expanding the impact and appreciation for...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...which had affected his judgement. Eventually, we published the paper in a high-impact journal and it worked...

... a neuroscience major, I was struck with the realisation that I didn’t want a career in neuroscience—whatever... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...towards a career in science communication, an established field of inquiry with which I was not yet familiar...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts... a scientific background and bring with them established interests and areas of expertise. Perhaps the... research explores risk-taking behavior among collision sport athletes, as well as the ethical and health...

...she would be looking at where I had published, who I had published with and whether I would be able to... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...Undergraduate sections and brought in more focus on the list of conferences I presented at and placed them under...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...

...and foster an effective working environment? Tom Ellis was offered a start-up package that included a postdoc... most surprising thing about becoming a PI? Tom Ellis has a one word answer: “Email. Nothing prepared ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...our latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend... lab!”   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...scientific research. These include policies for publishers and funders in the Transparency and Openness ...

... their precise positions within the plasmid. The list of enzymes that can be detected is 5x greater than...GenBank File or a SnapGene file for the sequences listed. You can then analyze the sequence in your sequence...Sequence” tabs. Features The table found here lists the common features detected in the plasmid sequence... map, direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...Analyzer guide A quick guide to all of the tabs listed above and their functions. We hope that these...

...recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast Light-mediated...transmission Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In...regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter podcast segment Discovered... CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding the...

...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...Commons. The North American firefly, Photinus pyralis, catalyzes a chemical reaction in its abdomen to...systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties of luciferase...Cofactors Secreted North American firefly (Photinus pyralis) Natural or codon-optimized 562 61 Luciferin ATP...Furimazine None Optional AkaLuc(derived from Photinus pyralis) Engineered   677 61 AkaLumine ATP No Luciferase...

... plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic control...by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment The neurons... system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently, the...and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable protein podcast segment ...

... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...