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We narrowed to 527 results for: MEA;

Showing: 181 - 200 of 527 results
  1. Plasmids 101: Screens vs. Selections

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    Blog Post
    ...question? If you’re thinking, ‘wait, those terms mean different things?!’, you aren’t alone! We’ve noticed...individually administered and then outcomes are measured. While the entire genome can be knocked out with...
  2. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ... yellow GECIs, OCaMP shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater...curves for OCaMP and various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal...
  3. Viral Vectors 101: Types of viruses

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    Blog Post
    ...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...
  4. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...generating mutant libraries exist, but none provide a means to continuously diversify all nucleotides within...size at the target site. The use of such libraries means that the diversity introduced by lambda red recombineering...
  5. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post
    ...conventional journal. Publishing via open access means that your manuscript will be available to anyone... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...
  6. Plasmids 101: Yeast Vectors

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    Blog Post
    ...yeast-specific origin of replication (ORI) and a means of selection in yeast cells, in addition to the ...some drawbacks to using auxotrophic markers as a means of selection: A specific selection marker needs...
  7. Transferable Skills Guide: Problem Solving

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    Blog Post
    ...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills” and...because I can resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing ...
  8. Addgene: The First Twenty Years

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    Blog Post
    ...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process...
  9. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this... E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used in vivo to study...
  10. dTAG - You're it!

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    Blog Post
    ... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal ...
  11. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...
  12. The time and cost required to make a plasmid

    Type
    Blog Post
    ...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96...
  13. To Each HIS Own

    Type
    Blog Post
    ...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the...
Showing: 181 - 200 of 527 results