We narrowed to 526 results for: MEA;
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TypeBlog Post... yellow GECIs, OCaMP shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater...curves for OCaMP and various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal...
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The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...respond as expected, use of representative images, measuring the main outcomes in several different ways, and...and you think there are four important times to measure that growth rate, you report the results of all... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post... for milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major...function well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...conventional journal. Publishing via open access means that your manuscript will be available to anyone... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers... -
Plasmids 101: Yeast Vectors
TypeBlog Post...yeast-specific origin of replication (ORI) and a means of selection in yeast cells, in addition to the ...some drawbacks to using auxotrophic markers as a means of selection: A specific selection marker needs... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this... E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used in vivo to study... -
dTAG - You're it!
TypeBlog Post... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal ... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...There are a variety of methods that can be used to measure your fluorescent protein’s propensity to aggregate...an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration 50% of the protein forms... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions... La Jolla California are not getting a raise. Meanwhile, in less densely populated places like the University... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be... -
The time and cost required to make a plasmid
TypeBlog Post...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96... -
To Each HIS Own
TypeBlog Post...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills” and...because I can resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing ... -
Addgene: The First Twenty Years
TypeBlog Post...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit. Part of that work meant facilitating the Material Transfer Agreement process... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...genomes/mL) can be measured by: Optical density: particle concentration is measured by looking at absorbance... -
6 Tips for Grant Writing
TypeBlog Post...this does not mean that you should apply for everything that’s out there, it just means that you shouldn... -
Plasmids 101: FLEx Vectors
TypeBlog Post...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...particles (VLPs). VLPs lack viral genomes which means they are non-infectious and non-replicative. Nanoblades...iPSCs and BM cells after Nanoblade treatment as measured by their maintenance of pluripotency marker expression... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...