We narrowed to 28 results for: MEA;

...Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements of membrane...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays....enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving spatial...ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic...sensors ZapCY1 and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum...di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger...Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in physiological range A genetically-encoded...

...(dark) acceptor, used for FLIM measurements Note that FRET measurements can be influenced by many factors...plasmids and resources for FRET experiments to measure protein interactions or conformation changes. Plasmid...use in FRET experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy ... and emission of a photon) of the donor can be measured using fluorescence lifetime imaging microscopy... multiphoton spectral imaging and its use for measuring resonance energy transfer mCerulean, mVenus, and... and Amber (mVenus Y67C) constructs for measuring energy transfer to multiple acceptors Koushik et al....

... two types of antigen specificity: monoclonal, meaning many copies of one clone that are specific to a... a certain epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes...bound. Signaling molecules , proteins that emit a measurable light or color in response to a specific stimulus...proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here, HRP is used as ...samples can be normalized to a total protein measurement. Both methods will also serve as a control for...conjugate, typically HRP, is activated and its output measured. This method can be performed using the indirect...Fix your samples. Prepare your samples using permeabilization, antigen retrieval, and/or sectioning. Block...

...with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard curve is ...intensities of both the antibody and impurities are measured and the ratio of the antibody protein content ...Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized, and labeled with the antibody and a species-specific...

...use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...window) is an energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI...protein. The apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer...

...amplification, and high-quality quality control measures such as titering, purity assessment, sterility...institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could...including purification, titration, and quality control measures. We have distributed preps around the globe and...

...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...reporter. Unless otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid ...

...based on the gRNA sequence without cleavage. This means proteins with other functions can be fused to a ...Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they will be referred...principle, the ability to induce base transversions means that any base-to-base conversion is possible with...CASTs can result in bidirectional insertions — meaning they may not be suitable for applications where...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

...examples of reporter plasmids that can be used for measuring infectivity and neutralizing activity are listed...mCherry and firefly luciferase to simultaneously measure the # of cells containing reactivated latent provirus...

...protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical...

...your gene "in frame" with the fusion protein. This means that the final product should be translated...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...

...generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is...around a bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent...

...application-specific validation and consistent quality control measures. We work with trusted partners and experts in ...

...CASTs can result in bidirectional insertions — meaning they may not be suitable for applications where...

...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

...function of any viral vector. Addgene performs QC measures which verify the identity of the viral vector,...

...suffix sequences are given for the coding strand , meaning that the suffix will need to be reverse complimented...

...from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting to ...

...Many viral plasmids are also self-inactivating, meaning that they can only complete one round of transcription...

...Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) Zika Don’t see your field...