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We narrowed to 526 results for: MEA;

Showing: 1 - 20 of 526 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...because it turns a dynamic process into a static measurement and it provides limited information about what...fluorescent autophagy reporters now allows for the measurement of autophagic flux, or the changes in autophagic...strength excited at 586 nm to 440 nm serves as a measure of autophagy induction: when Keima is in the cytosol...is high. When untargeted, Keima can be used to measure bulk autophagy, but the addition of a mitochondria-targeting...3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an ...18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy in mammalian...Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal of molecular...

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...organic solvents’ impact on lipids means that they are able to permeabilize your sample while fixing it, which...Examples of different permeabilization strategies and when to use them. A) Not permeabilizing is suitable for... first couple of steps - mainly, fixing and permeabilizing. Fixation What is the point?  Fixation allows...the protein. Created with BioRender.   Permeabilization What is the point?  Antibodies are large proteins...they are not able to diffuse across natively. Permeabilization essentially punches holes in the cell membranes...In fact, you may get cleaner results by not permeabilizing as you’ll prevent binding to any target protein...of the cell. However, assuming you do need to permeabilize, you have a few options for what reagents to...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...viral vector solution, and generally these methods measure different characteristics of the solution. These... containing viral genomes. Physical titers are measured by quantifying the concentration of viral genomes... it is important to understand what these values mean and how they can be compared.   Figure 2:...circled in red) report physical titer, which is measured by qPCR.   Physical titer is typically calculated...repeating the exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more...Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because (physical) AAV titers...

  4. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...Microvolume spectrophotometers make it quick and easy to measure sample concentration and purity using only a tiny... take a moment to review what exactly you are measuring and how to interpret your results.  Quick note...vary.  How it works — briefly Spectrophotometers measure how light travels through a sample — in this case...to those of pure DNA (Figure 1D). This property means that even samples with quite high levels of phenol...they can impair your ability to get an accurate measure of DNA concentration. This is especially problematic... Take multiple readings to average out noisy measurements. Be cautious when interpreting purity ratios...buffer you use will also affect how accurate your measurements are. While water is commonly used for elution...

  5. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...important for regulating signal transduction and measuring kinase activity at the single-cell level can aid...whose cellular localization serves as a proxy measurement of kinase activity. The key advantage of KTRs...Advantages of KTRs When compared to FRET reporters for measuring kinase activity, KTRs have two key advantages....nucleus, while FRET-based reporters can be used to measure kinase activity in specific subcellular compartments... Regot, S., & Covert, M.W. (2018). Live-cell measurements of kinase activity in single cells using translocation...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements of membrane...of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays....enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving spatial...ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic...sensors ZapCY1 and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic reticulum...di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger...Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in physiological range A genetically-encoded...

  7. Antibody Guide

    Type
    Collection
    ... two types of antigen specificity: monoclonal, meaning many copies of one clone that are specific to a... a certain epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes...bound. Signaling molecules , proteins that emit a measurable light or color in response to a specific stimulus...proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here, HRP is used as ...samples can be normalized to a total protein measurement. Both methods will also serve as a control for...conjugate, typically HRP, is activated and its output measured. This method can be performed using the indirect...Fix your samples. Prepare your samples using permeabilization, antigen retrieval, and/or sectioning. Block...

  8. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...energy transfer is measured using fluorescence microscopy by exciting the donor and measuring the emission ...collection of plasmids suitable for FRET experiments to measure protein interactions or conformation changes, as...calibrate the numerous and disparate methods used to measure FRET. Plasmid FRET Pair C5V Cerulean attached to...

  9. Antibody Production

    Type
    Collection
    ...with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard curve is ...intensities of both the antibody and impurities are measured and the ratio of the antibody protein content ...Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized, and labeled with the antibody and a species-specific...

  10. Promega Plasmid Collection

    Type
    Collection
    ...use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...window) is an energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI...protein. The apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer...

  11. AAV Packaged on Request

    Type
    Collection
    ...amplification, and high-quality quality control measures such as titering, purity assessment, sterility...institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could...including purification, titration, and quality control measures. We have distributed preps around the globe and...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...reporter. Unless otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid ...

  13. CRISPR Guide

    Type
    Collection
    ...based on the gRNA sequence without cleavage. This means proteins with other functions can be fused to a ...Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes they will be referred...principle, the ability to induce base transversions means that any base-to-base conversion is possible with...CASTs can result in bidirectional insertions — meaning they may not be suitable for applications where...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...your gene "in frame" with the fusion protein. This means that the final product should be translated...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...

  16. Bacterial Expression Systems

    Type
    Collection
    ...generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is...around a bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent...

  17. Antibodies

    Type
    Collection
    ...application-specific validation and consistent quality control measures. We work with trusted partners and experts in ...

  18. Fluorescent Protein Guide

    Type
    Collection
    ...Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals, cells, or groups...

  20. Optogenetics Plasmid Collection

    Type
    Collection
    ...as microbial opsin and optical switch tools to measure and manipulate cells and their biomolecular processes...
Showing: 1 - 20 of 526 results