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We narrowed to 538 results for: PAN

Showing: 181 - 200 of 538 results

  1. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first...

  2. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...other disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

  3. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...career?”, "Have they sought out opportunities to expand their skill sets?", “Have they succeeded in what...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What...general research community. Phillips Petroleum Company first commercially developed K. phaffii in the ...Since then, multiple biotech and pharmaceutical companies (along with some research laboratories) have picked...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... in our first study, but we're planning to soon expand to other cell types, including more physiologically-relevant...we’ve all been affected in various ways by the pandemic, it is clear that we have to stick together (6...Addgene, who will be taking over the distribution to expand the network of scientists working on this even ...

  6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...into a plasmid, you can send your plasmid to a company. For a few hundred dollars, they will inject the... your strategy and the services offered by the company, you might have to map into which chromosome the...can be dissected at various times throughout the lifespan of the fly to determine the implications of your...

  7. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ... the project. I was invited to participate on a panel about “Social Media for Science Communication” at... know how I was selected to participate on this panel, but my consistent writing for the Addgene blog ...Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers...

  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they’re left ...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...you’ll likely be able to create a small, usable panel.  First, fluorophores vary in brightness. You don...

  10. Clear and Accessible Writing

    Type
    Blog Post
    ...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about...Figure 1, Addgene chose this alt text: "The left panel is a profile photo of an iodixanol gradient after...passage of supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...

  11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

  12. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...previous blog post about how they started their company here. The first transgenic plant was engineered...distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There are already...the years were generated by large agricultural companies and carry associated intellectual property concerns...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    ...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...

  15. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...therapeutic and vaccine design. We all want tools that expand what we can achieve, but are simple to pick up ... with SpyTag/SpyCatcher, allows for iterative expansion DogTag/DogCatcher SpyTag/SpyCatcher Rapid covalent...nature’s building blocks. Applications are quickly expanding with these kinds of Click Biology reactions. For...

  16. 6 Tips for Grant Writing

    Type
    Blog Post
    ...someone senior from accounts or HR on the judging panel. It’s important for this person to get a clear and...scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities to attend...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible adults... this technology and angry at the agriculture companies that produce GMO food plants. When a press piece... of our business, because we are building this company, and that’s what we want it to do. It’s exhilarating...

  18. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...know if you will need to compensate in your flow panel, you will need to know both the fluorescent markers...positive APC population is circled in red in both panels.    Pro tip! To determine which way your population...compensation between APC and APC-Cy7, with compensation panels below each plot.  The general rules of compensation...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...without the financial and logistical stress that accompanies traveling for campus visits. This is definitely... technologies that helped us navigate a global pandemic can also be the thing that provides a new perspective...developed virtual environment resulting from the pandemic, students who are unable to attend in-person interviews...

  20. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...vivo HSC expansion cultures. A) Experimental design. B) UMAP of cell states in ex vivo expansion cultures...Meghan Rego Attention AAV users: in an effort to expand your gene delivery toolkit, Addgene now offers ...
Showing: 181 - 200 of 538 results