We narrowed to 535 results for: PAN

... in our first study, but we're planning to soon expand to other cell types, including more physiologically-relevant...we’ve all been affected in various ways by the pandemic, it is clear that we have to stick together (6...Addgene, who will be taking over the distribution to expand the network of scientists working on this even ...

...into a plasmid, you can send your plasmid to a company. For a few hundred dollars, they will inject the... your strategy and the services offered by the company, you might have to map into which chromosome the...can be dissected at various times throughout the lifespan of the fly to determine the implications of your...

...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

...your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they’re left ...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...you’ll likely be able to create a small, usable panel.  First, fluorophores vary in brightness. You don...

...situation is this more true than the current global pandemic in which scientists race to find vaccines, therapeutics...to help find a faster solution to the COVID-19 pandemic. Though we’ve confirmed the expected features... science move faster in the middle of a global pandemic. And the reach of these plasmids is indeed global...

...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about...Figure 1, Addgene chose this alt text: "The left panel is a profile photo of an iodixanol gradient after...passage of supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...

... the project. I was invited to participate on a panel about “Social Media for Science Communication” at... know how I was selected to participate on this panel, but my consistent writing for the Addgene blog ...Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers...

...previous blog post about how they started their company here. The first transgenic plant was engineered...distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There are already...the years were generated by large agricultural companies and carry associated intellectual property concerns...

...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...

...therapeutic and vaccine design. We all want tools that expand what we can achieve, but are simple to pick up ... with SpyTag/SpyCatcher, allows for iterative expansion DogTag/DogCatcher SpyTag/SpyCatcher Rapid covalent...nature’s building blocks. Applications are quickly expanding with these kinds of Click Biology reactions. For...

...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol...

...someone senior from accounts or HR on the judging panel. It’s important for this person to get a clear and...scoring system or meeting with their grant submission panel. Some funding bodies provide opportunities to attend...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

...Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible adults... this technology and angry at the agriculture companies that produce GMO food plants. When a press piece... of our business, because we are building this company, and that’s what we want it to do. It’s exhilarating...

...know if you will need to compensate in your flow panel, you will need to know both the fluorescent markers...positive APC population is circled in red in both panels.    Pro tip! To determine which way your population...compensation between APC and APC-Cy7, with compensation panels below each plot.  The general rules of compensation...

...without the financial and logistical stress that accompanies traveling for campus visits. This is definitely... technologies that helped us navigate a global pandemic can also be the thing that provides a new perspective...developed virtual environment resulting from the pandemic, students who are unable to attend in-person interviews...

...vivo HSC expansion cultures. A) Experimental design. B) UMAP of cell states in ex vivo expansion cultures...Meghan Rego Attention AAV users: in an effort to expand your gene delivery toolkit, Addgene now offers ...

...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University...

...hypotheses being rejected, etc.), while reminding participants of the value of this approach and encouraging...by making a greater number of students active participants in the research process – so if this sounds ...classroom-based undergraduate research experiences spanning chemical biology, biochemistry, and neurobiology...

...is ideal for tracking large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D... time. 1D barcodes can be purchased from many companies such as Corning. Tubes can even have specific ...specific barcodes etched or printed onto them by companies such as Tubewriter. Any lab can benefit from using...

...Nucleocapsid Protein antibodies #211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against... against vinculin as a loading control (right panel). Image from Addgene. These antibodies are recombinant...