We narrowed to 257 results for: PORA

1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ...different spike protein variants that can be incorporated into pseudoviruses. The lab used these pseudoviruses...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

3. Hot Plasmids February 2024

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   ...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection...

4. Plasmids 101: FLEx Vectors

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   ...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...

5. Tips for Titering Your Lentiviral Preps

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   ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

6. Experimenting in an Open Source lab: from CRISPR to Cats

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   ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

7. How-to: 5 Steps to a Great Panel Discussion

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   ... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ... up for real: bank account, business cards, incorporation, laboratory lease, etc. I still flip to that...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...the likelihood that donor DNA will interact with sporadic double-stranded chromosomal DNA breaks “that always...

10. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009): 1863-1868...

11. Plasmids 101: Knockout/Knock-In Plasmids

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    ...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...incorrectly and may function improperly. 3. Incorporation into a FRET biosensor A FRET biosensor is a ...

13. Light Sheet Fluorescence Microscopy

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    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

14. Plasmids 101: Multicistronic Vectors

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    ...single transcript. The two strategies most widely incorporated into plasmids for research purposes are described...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...

17. A Guide to Starting Your Own Journal Club

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    ...yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting or perhaps...

18. SciComm: Taking Science to Elementary and Middle Schools

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    ...has made strong efforts in the last 5 years to incorporate more science and technology into the school curriculum...

19. Antibodies 101: Producing Recombinant Antibodies

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    ...for further processing. It may be helpful to incorporate regular feeds during this incubation time to ...

20. Summer SciComm Series: Modes of Communication

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    ...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...