We narrowed to 6 results for: PORA

1. CRISPR Library Amplification

   Type

   Protocol

   ... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents.../or increasing the skewness of the pool. 8 electroporation cuvettes (BioRad, Micropulser, 0.1 cm ) 20 ...DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use...Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud pop often ...accompanied by some light in the cuvette during the electroporation step. I got a heavy recombined band by digest...

2. Water Bath Protocol

   Type

   Protocol

   ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

3. Protocol - Bacterial Transformation

   Type

   Protocol

   ... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

4. Gibson Assembly Protocol

   Type

   Protocol

   ...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed...

5. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

6. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...