... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents.../or increasing the skewness of the pool. 8 electroporation cuvettes (BioRad, Micropulser, 0.1 cm ) 20 ...DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use...Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud pop often ...accompanied by some light in the cuvette during the electroporation step. I got a heavy recombined band by digest...

...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed...

...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...