We narrowed to 255 results for: PORA

...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo...- or reward-related behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors...

...tRNA is incorporated to the growing peptide chain. To expand the genetic code and incorporate a ncAA into...by reassigning specific codons to enable the incorporation of amino acids beyond the 20 standard ones, ... cell, the ncAA provided in the media can be incorporated using the existing protein translation machinery... tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid often carries multiple tRNA...target plasmids in genome code expansion for the incorporation of non-canonical amino acids (ncAA) in a protein...modified codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI Return...modified to enhance non-canonical amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all ...

...CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C...Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI CACNA1A GFP U6, Cbh Spinocerebellar...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1...

...generally perform by chemical transfection or electroporation. Expression can be transient, or you can generate... to target cells through microinjection or electroporation. The target cells translate the mRNA encoding...delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA... breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base and prime...29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents.../or increasing the skewness of the pool. 8 electroporation cuvettes (BioRad, Micropulser, 0.1 cm ) 20 ...DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use...Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud pop often ...accompanied by some light in the cuvette during the electroporation step. I got a heavy recombined band by digest...

...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells. Angew Chem...or Cre-dependent) A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics...HT3.0 Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566...lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1...

... has developed several tools useful for the incorporation of phosphoserine into your gene of interest.... where it recognizes the UAG amber codon and incorporates phosphoserine into the growing polypeptide chain...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...

...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

...instability and cell death. It is important to incorporate appropriate controls such as Cre-only lines to...top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression vector...

...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

...the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription. The edited...

...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...

...Grefen Lab. FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag ...

...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...

...The vast majority of cases (90–95%) of ALS are sporadic, having no prior family history. A small percentage...

...NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature cell targeting...

... the expression cassette of interest is incorporated into the host DNA. For mammalian cells, you ...

...nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop...

...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...

...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed...