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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 257 results for: PORA

Showing: 1 - 20 of 257 results

  2. CRISPR Library Amplification

    Type
    Protocol
    ... mix. Electroporate cells (one at a time for a total of eight electroporations): Electroporator Conditions...and have 1 mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents.../or increasing the skewness of the pool. 8 electroporation cuvettes (BioRad, Micropulser, 0.1 cm ) 20 ...DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use...Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud pop often ...accompanied by some light in the cuvette during the electroporation step. I got a heavy recombined band by digest...

  3. Genetic Code Expansion

    Type
    Collection
    ... the codon), the amino acid from the tRNA is incorporated into the growing peptide chain. To expand the...aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid ...the cells, the non-canonical amino acid can be incorporated using the existing protein translation machinery...translation machinery are needed in order to incorporate a non-canonical amino acid into the protein of... the place where the new amino acid would be incorporated. Other options, such a 4-base pair codons, have...modified to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all ...function removed, genomic deletions for improved incorporation Michael Jewett 69495 rEc.13.delA 13 amber sites...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... 10 6 cells in 100 μl of electroporation solution and add to electroporation cuvette. Add 5 μg of each...construct. Electroporate cells with 250 volts for 5 msec in a 2 mm cuvette using an electroporation system...of culture media after electroporation. Minimize the time between electroporation and transferring the ...involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described in detail for...Li D, Li J. Cell Stem Cell . 2013. PubMed . Electroporation: theory and methods, perspectives for drug ...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI CACNA1A GFP U6, Cbh Spinocerebellar...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1..._Paragranulin-no linker GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176918 pcDNA3.1...pcDNA3.1(+)_Granulin 1+linker2 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176919 pcDNA3.1...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells. Angew Chem...or Cre-dependent) A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics...HT3.0 Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566...lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1...

  7. Water Bath Protocol

    Type
    Protocol
    ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

  8. Worm Expression Resources

    Type
    Collection
    ...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... has developed several tools useful for the incorporation phosphoserine into your gene of interest....able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...

  10. Plan Your Experiment

    Type
    Collection
    ... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

  11. Cre-lox system

    Type
    Collection
    ...activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The promoter...Expression of Transgenes Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32....

  12. Adenovirus Plasmids

    Type
    Collection
    ...plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule...

  13. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription. The edited...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed...

  16. Viral Vectors

    Type
    Collection
    ...for long term studies) while other viruses are temporary in the host. Use this guide to learn more about...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...

  19. CRISPR Guide

    Type
    Collection
    ...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...
Showing: 1 - 20 of 257 results