...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...photoswitchable” proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185...

...cells, they kill off any cells that have not incorporated the lentiviral genome and those cells that survive...expanded to create stable cell lines, which have incorporated the lentiviral genome and harbor the genetic...interest and contains the sequences that will incorporate into the host cell genome, but cannot produce...transcription of the full-length virus after it has incorporated into a host cell. The potential for oncogenesis...

...vitro DNA replication but relies on the random incorporation of modified, fluorescently tagged bases onto...difference in this process occurs when the polymerase incorporates a fluorescently tagged nucleotide. Because these...halted once the fluorescently tagged base is incorporated. Sanger sequencing requires a lot of DNA because...

... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...

...the screening process. Amplify the library (electroporation and maxiprep). If delivering as virus, make...

...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...