We narrowed to 10 results for: PORA

...generally perform by chemical transfection or electroporation. Expression can be transient, or you can generate... to target cells through microinjection or electroporation. The target cells translate the mRNA encoding...delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA... breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base and prime...29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

...neural populations at distinct times. Temporal considerations Temporal precision is key in optogenetic experiments...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...photoswitchable" proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...desynchronization of neural activity, with single-spike temporal resolution. PloS One, 2 (3), e299. https://doi.org...

...transcription of the full-length virus after it is incorporated into a host genome. This is often found in second-generation...cells, they kill off any cells that have not incorporated the lentiviral genome and the cells that survive...interest and contains the sequences that will be incorporated into the host cell genome but cannot produce...after one round of reverse transcription. After incorporation into a host cell, this deletion prevents further...

...cells, they kill off any cells that have not incorporated the retroviral genome and the cells that survive...interest and contains the sequences that will be incorporated into the host cell genome but cannot produce...after one round of reverse transcription. After incorporation into a host cell, this deletion prevents further...

...Common Bacterial Promoters Researchers often incorporate the following promoters into plasmids to drive...Common Eukaryotic Promoters Researchers often incorporate the following promoters into plasmids to drive...

... 3). Sanger sequencing relies on the random incorporation of modified, fluorescently-tagged bases during...denoted as ddNTPs), halting synthesis after their incorporation. Sanger sequencing requires a lot of DNA because...

...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...

...the same basic process: Amplify the library (electroporation and maxiprep). If delivering as virus, make...

...first-generation rAdV vectors than later generations, which incorporate additional genomic deletions. Figure 2: First-generation...

...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...