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We narrowed to 653 results for: PPL

Showing: 181 - 200 of 653 results

  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...pre-form primary-secondary antibody complexes before applying them to samples, such as when performing IHC in...can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are the same species...fragments described above can be used for similar applications and have similar advantages, though with some...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...tools available for bacteria and some of the applications for which they’ve been used. The beginnings ...the specific gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes... E. coli. Furthermore, to demonstrate wider applicability they demonstrated success of Cas9 mediated repression...scenario allows for several potential CRISPR applications. First, CRISPR can be used to target locations...resolve them, and the cells die.  This could be applied to diseases where the presence of just a single...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    ..., its dimeric nature can interfere with some applications. Several labs have been working to produce monomeric... for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038...proven itself a good option for essentially all applications. Addgene’s plasmid collection includes over ...clone (M2) has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...polyclonal antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are ...

  5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...reproducibly labeled retinal cells can be found here.  Applications for synthetic promoter AAVs Besides restricting...synthetic promoters have a few other potential applications: Using promoter combinations for targeted expression... promoters expressed in. The lab tested this application using one promoter to drive expression of Cre-mCherry... rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling would...

  6. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...end-users, size of the transgene, and downstream applications should all be considered when choosing the correct...procedure for a single 10 cm2 dish can be easily applied to multiple dishes, remember that handling multiple... as resources available, cost and downstream applications. For example, while the PEG precipitation method... When assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system and...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. In addition, they generated...activation triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...surfaces that come into contact with the rAAV and apply common cell culture practices diligently throughout...which the required adenoviral helper genes are supplied on two different packaging plasmids: the pHelper...al., 2010). For certain in vitro and in vivo applications, you can consider using unpurified AAV from ..., 2017). You can learn more about common AAV applications by reading our Viral Vectors 101 series!  This...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...your resume pulled to the top of the pile when you apply for such positions. Of course, if your goal is to...transition from an academic setting—especially if job applications require teaching experience or high numbers ...s program. Once you’ve found a few positions to apply for, compare and contrast industry postdocs at those...background in cell and developmental biology and is applying this towards researching neurodegenerative diseases...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... Association (PTA) has been instrumental in supplementing that curriculum. It’s through the PTA that I...Spangler Science The Fab Lab Middle school: supplementing the science curriculum Sharing my expertise ...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....you want to make sure their STEM education is supplemented. A 2009 study by the NSF showed that among US...

  12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...used for most of the current genome engineering applications of CRISPR including: generating single or multi-gene...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required...this technique in cell culture, it has also been applied to genome editing of mouse zygotes, through a process... of eventually using this method for in vivo applications. This technique utilizes Cas9 proteins harboring...

  13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...down to the exact plasmid you need. The ability to apply a wide range of filters isn’t new to Addgene’s plasmid... it’s also a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ ...consider. If you get this far and realize you need to apply different filters you do not need to start a new...” plasmids returns more than 1300 results, and applying a filter for “CRISPR” plasmids keeps more than...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... plasmid or group of plasmids' functions and applications. We hope that these articles make it easier ...FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power of mCyFP1, the Lin... Coselection efficiency is so high that some applications may permit working with edited “pools” of cells... subtilis has a fully sequenced genome, many applications in biotech, and is considered the most studied...regulation to genome engineering that enables new applications, such as reversing the effects of dCas9 binding...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...requiring people who are willing to quickly learn, and apply their knowledge to a variety of problems. In this...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients manage...literature, and critical analyses skills were applicable to my cases. I can directly use my understanding...the gap between the science and the commercial applications of a particular therapy. With your scientific...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...you. One such option is the CRISPR Design tool supplied by the Zhang lab. This simple website invites ...the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...you can order them yourself through whatever DNA supply company you currently use. You can also find many...selected gRNA’s, and has the bonus benefit of supplying you with primers for validating your edit in mice...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage tracing in other systems...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving... Currently, Brainbow techniques are also being applied to study model organisms such as Drosophila and...brain and other biological systems. Interested in applying Brainbow to your research? Check out Brainbow ...

  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...

  20. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... closely read the publication, including the supplemental data. You’re most likely to find thorough characterization...spectral and kinetic activation information, in the supplemental data instead of in the main text.  Then, check...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...
Showing: 181 - 200 of 653 results