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We narrowed to 651 results for: PPL

Showing: 181 - 200 of 651 results

  1. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...scientific aspects of writing. Following his advice, I applied to the class. Receiving the email that I had been...the ASM headquarters. I decided to go ahead and apply; thinking I probably would not get selected for ...knowledge with practical understanding of how to apply it. The personal interaction of critiquing others...well as being critiqued added an extra level of application not present in the online class.   Four ways ...

  2. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in... same species as your primary/sample, and then applying this mixture to your tissue (Tuson, et al., 1990...After washing away unbound fragments, you can then apply your primary and secondary antibodies as normal....Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  3. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... solutions that facilitate most experimental applications in CRISPR engineering. When compared to that...easy to fault the lack of features in a single application. The question is then, should we use all tools...categories. On the one hand we have software tools that apply some approximate or sensible rules to determine ...This often results in software design that is applicable to only one, or a subset of organisms. In only...ProtospacerWB was developed with the purpose of applying CRISPR technologies to new organisms and will ...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    ...antibodies may just not perform as well in one application as another, so it is always best to have options...for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more...fluorescent proteins (FPs) necessary for many of these applications have lagged. Fixation, chemical staining, and...well-suited for the demands of advanced microscopy applications such as super resolution, electron, and expansion...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... our knowledge. Disease Applications Q13: Does AAV have potential applications in diseases which may be...1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/...CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area is discussed...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry... more, take a look at their 551 page e-book on applying MIQE guidelines! With these kinds of resources...melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April ...Solinas A; Booth J; Brown T. Mode of action and application of Scorpion primers to mutation detection, Nucleic...

  8. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...pre-form primary-secondary antibody complexes before applying them to samples, such as when performing IHC in...can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are the same species...fragments described above can be used for similar applications and have similar advantages, though with some...

  9. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...tools available for bacteria and some of the applications for which they’ve been used. The beginnings ...the specific gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes... E. coli. Furthermore, to demonstrate wider applicability they demonstrated success of Cas9 mediated repression...scenario allows for several potential CRISPR applications. First, CRISPR can be used to target locations...resolve them, and the cells die.  This could be applied to diseases where the presence of just a single...

  10. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...end-users, size of the transgene, and downstream applications should all be considered when choosing the correct...procedure for a single 10 cm2 dish can be easily applied to multiple dishes, remember that handling multiple... as resources available, cost and downstream applications. For example, while the PEG precipitation method... When assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system and...

  11. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

  12. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...reproducibly labeled retinal cells can be found here.  Applications for synthetic promoter AAVs Besides restricting...synthetic promoters have a few other potential applications: Using promoter combinations for targeted expression... promoters expressed in. The lab tested this application using one promoter to drive expression of Cre-mCherry... rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling would...

  13. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...polyclonal antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are ...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    ..., its dimeric nature can interfere with some applications. Several labs have been working to produce monomeric... for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038...proven itself a good option for essentially all applications. Addgene’s plasmid collection includes over ...clone (M2) has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here...

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean... wild type (WT), two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv...Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. In addition, they generated...activation triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...surfaces that come into contact with the rAAV and apply common cell culture practices diligently throughout...which the required adenoviral helper genes are supplied on two different packaging plasmids: the pHelper...al., 2010). For certain in vitro and in vivo applications, you can consider using unpurified AAV from ..., 2017). You can learn more about common AAV applications by reading our Viral Vectors 101 series!  This...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...your resume pulled to the top of the pile when you apply for such positions. Of course, if your goal is to...transition from an academic setting—especially if job applications require teaching experience or high numbers ...s program. Once you’ve found a few positions to apply for, compare and contrast industry postdocs at those...background in cell and developmental biology and is applying this towards researching neurodegenerative diseases...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... Association (PTA) has been instrumental in supplementing that curriculum. It’s through the PTA that I...Spangler Science The Fab Lab Middle school: supplementing the science curriculum Sharing my expertise ...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....you want to make sure their STEM education is supplemented. A 2009 study by the NSF showed that among US...

  19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...used for most of the current genome engineering applications of CRISPR including: generating single or multi-gene...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required...this technique in cell culture, it has also been applied to genome editing of mouse zygotes, through a process... of eventually using this method for in vivo applications. This technique utilizes Cas9 proteins harboring...

  20. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...down to the exact plasmid you need. The ability to apply a wide range of filters isn’t new to Addgene’s plasmid... it’s also a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ ...consider. If you get this far and realize you need to apply different filters you do not need to start a new...” plasmids returns more than 1300 results, and applying a filter for “CRISPR” plasmids keeps more than...
Showing: 181 - 200 of 651 results