We narrowed to 749 results for: REV

...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

...Collection Viral Vector Packaging Service Content last reviewed: 4 December 2025 Do you have suggestions for other...

...real, peer-reviewed study. The second group read the same abstract but with the names reversed, meaning ...looked at how people perceive and evaluate peer-reviewed sources of information if those sources affirm...meaning that the findings of the study were also reversed. The third group read a version of the abstract where...

...of the fluorescent protein dsRed that changes irreversibly its color from green to red when it is oxidized...et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation using SpyLigase Building...excitation of neurons. The paper describes five previously unknown channelrhodopsins from different species...designed two vectors for use in conditional and reversible gene silencing in RNAi transgenic mouse models...Jesse Rinehart and Dieter Söll at Yale University previously changed the way researchers can explore important...engineering demonstrate improved efficiency over previous reports. For additional information on using ...

...Blog: Chemogenetics vs. Optogenetics Content last reviewed: 25 September 2025 Do you have suggestions for...

...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

...reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying CRISPR/Cas-...target region into the N-terminus of mCherry. To prevent alternative start site initiation, the lab removed... this one mutated methionine was not enough to prevent alternative initiation, so they mutated the methionines..., Davidson NO, Madison BB (2017) A stable but reversible integrated surrogate reporter for assaying CRISPR...

...identified have little sequence similarity to previously identified Cas13a-c orthologs, but they do include...system. Since Cas13d is functionally similar to previously discovered Cas13 enzymes - what makes these orthologs...80% for other Cas13a and Cas13b effectors. Like previously characterized Cas13 enzymes, CasRx also displays... manipulate RNA species through exon skipping. Previous CRISPR exon-skipping approaches used two guide...

... pR, preventing expression of a reporter gene. When light is present, YFI is inactive, preventing repressor...inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...

...and exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo...increased sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP...described in Suzuki et al. improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color... its substrate furimazine. In addition to the previously described applications, the enhanced signal of...

...lot of trial and error.” The structural details revealed two exposed RNA loops, which Konermann recognized...of all, the Cas9s can be turned on and off, to reversibly activate or repress genes of interest. They also...in your experiments, Addgene recommends that you review the steps for how to use pooled lentiviral CRISPR... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

...treatment regimen and were not preexisting. This was previously debated in the cancer field and was difficult...be amplified using the same sets of forward and reverse primers). The abundance of a particular barcode...replicate; prior to treatment, there are small (previously undetectable) populations of cancer cells that...that combination treatment with multiple drugs reveals even smaller preexisting populations with resistance...

...In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...and the region of DNA between the loxP sites is reversed.   Deletion: If the sites face in the same direction... cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-...

...Blog: Chemogenetics vs. Optogenetics Content last reviewed: 25 September 2025 Do you have suggestions for...

...Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025...

...low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more ...uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout...completed in just 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs... this toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used...

...fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo et al. Nat Commun. 2019...options. While the BiFC interaction is typically irreversible and requires some time for chromophore maturation...maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides maximum...important for their interaction. You can read our previous blog post for more on BiFC principles and experimental...

..., you will need to correct it). It’s easy for reviewers to spot when an application is rushed (whether...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) to... times and will have useful advice about what reviewers in your area are looking for. Remember though,...for grant success The two most important things reviewers will be looking for in your research proposal ...

...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

...our expert viral vector production team carefully review your cargo/serotype selection after you submit ...