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Addgene

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We narrowed to 536 results for: SHI

Showing: 181 - 200 of 536 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of...read the Addgene interview with Mark Howarth. Shining a light on channelrhodopsins – Chronos & Chrimson...spectral peak at 590 nm, which is 45 nm more red-shifted compared to other variants. The group further optimized...functional GFP, and the cells will turn green. Mashiko et al., Sci Rep 2013 Nov 27; 3:3355. Rinehart...TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN assembly ...

  2. Summer Fun at Addgene!

    Type
    Blog Post
    ...and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions via blog post... the next few weeks.)  Finally, Addgenies love sunshine and warm weather - check out our Instagram all...endeavors - in between some much-deserved vacations, sunshine, and beach trips, of course.    *not me, though...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ... FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput and...example, offers automated, benchtop, high throughput washing devices that are compatible with a variety of pipette...incentives (for example, by grant agencies), we can shift toward a more sustainable one. The math on this ...

  4. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There...coding sequences often result in loss-of-function frameshift mutations. However these may require restriction...genomic landscape may be surveyed in unprecedented fashion. Targeted deletions are just one of many available...

  5. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...repository in 2023.  In June, we announced a new partnership with the Institute for Protein Innovation (IPI...we’re pleased to announce that we are now able to ship rabies plasmids in our repository to international...through active conversation and collaboration.  Wishing you a healthy, productive, and thriving 2024, mirroring...

  6. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...muscular atrophy patients. Noushin Nabavi - Peritoneal Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow...diagnostic tools and animals models of the disease in partnership with Taconic Biosciences. The study of peritoneal...

  7. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...like something out of your nightmares, shared ownership of materials produced from your collaboration ...the project. The state of research is constantly shifting, and long-distance collaborations are becoming...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...deaminase from sea lamprey fused to Cas9 nickase (Nishida et al., 2016). Target-AID acts similarly but not...efficiency but higher indel formation than BE2 Nishida et al., 2016 Target-AID   Edits 3-5 base window...685. https://doi.org/10.1038/s41587-022-01533-6 Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto...Mochizuki, M., Miyabe, A., Araki, M., Hara, K. Y., Shimatani, Z., & Kondo, A. (2016). Targeted nucleotide editing...

  11. The Strength of Story Telling

    Type
    Blog Post
    ...whether I’m working with a talented journalist to shine light on living as a scientist in Beijing, talking...emotional investment and following cause-and-effect relationships are all things almost all of us are programmed...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...

  13. The MTA at Addgene

    Type
    Blog Post
    ...returned the MTA, Addgene can move forward with shipping the material to the requestor.  Why the MTA?  ...certainly frustrating to find out your plasmid hasn’t shipped because a form needs to be signed. But the truth... And that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...FDA-approved). This is a big job, but under the leadership of Nevan Krogan at UCSF we’ve brought together...line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members...Foussard, and Dave Gordon) have been quite busy shipping and sending plasmids to all who request them -...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... for clear communication, fair credit through authorship, and acceptance of a diversity of work styles...stress the fact that “miscommunication, a lack of leadership, and inappropriate tools or writing approaches...that was probably lost during these weird times of shift working and social distancing. The take home message...

  16. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...jumped at the chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his...clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM paper – ...panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz...

  17. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...however, it requires high doses. Requests have shifted toward the more efficient AAV-PHP.eB, developed...Evolutionary tree depicting the phylogenetic relationship of common research species. Among these species..., Kim, H.M., Rajendran, P.S., Tompkins, J.D., Shivkumar, K., Deverman, B.E., Gradinaru V. Systemic AAV...0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin....017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018)....

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...launched the BioBuilder Educational Foundation in partnership with high school teachers to boost interest and...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...economically disadvantaged students for paid summer internships. A summer teacher professional development program...

  20. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...is a co-founder of ComSciCon and Chair of its Leadership Team.  Nathan did his PhD in astronomy and works...
Showing: 181 - 200 of 536 results