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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 11 results for: SHI

Showing: 1 - 11 of 11 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...determine the best concentration for your assay. Washing steps can be done by hand but if available, use...pipette to add 200 µL wash buffer to each well. Washing can be done by hand but if available, use an automated...

  2. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...comfortable shoes that cushion your feet. Eye protection such as goggles and/or face shields can be used as needed...Close-toed shoes Gloves Eye protection and face shields, as needed Guidelines Your lab coat should be buttoned...

  3. Ligation Independent Cloning

    Type
    Protocol
    ...stop the exonuclease function of the enzyme and shift its activity back to polymerase. In our case, dGTP...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...scrape and use spreader to scrape plates. Pro-Tip Pushing motion is better than pulling motion Take care ...
Showing: 1 - 11 of 11 results