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We narrowed to 660 results for: SIM

Showing: 181 - 200 of 660 results

  1. RUBY-Red Siliques

    Type
    Blog Post
    ...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed...

  3. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...provides a combinatorial indexing strategy that simultaneously tracks clonal history and cell identity at ...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...

  4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality of the...of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex control via scRNA...Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable control of gene...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ... about operating the rest of the car as well. Similarly, cell free expression systems contain all the ...students to use in the classroom because the students simply need to add water to express a variety of proteins...Addgene.org Browse our protocol videos to learn simple lab techniques Check out our molecular biology ...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...disappears from the video, and prepares a coffee, or simply moves away from the desk to switch off a few minutes...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

  9. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...amplification protocol, but the overall workflow is similar for most libraries. Here are the key points you... For libraries where the plasmids are all very similar sizes, like a gRNA library, this is less of a concern... hope you’ll agree with me that they look very similar to each other - that’s exactly what we want! At...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, ...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt...

  11. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...facilitates the reproduction of the structure in a similar way to the biological protocol, and assembly designs... young movement of sharing open hardware has a similar goal. The sharing of modules, ideas, and blueprints...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and...

  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs, ...mammalian system. Since Cas13d is functionally similar to previously discovered Cas13 enzymes - what makes...their cleavage pattern: EsCas13d produced very similar cleavage products even when guides were tiled across...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    ...create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...

  14. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Identifying the Issue You can’t fix an experiment by simply running it unchanged over and over again, and the...was not trying even harder to start earlier, but simply moving all my prep work to the day before, which...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

  15. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...conjugate that can be activated to emit light with a simple kit, and is readily available on any number of ...ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive ... this is an Antibody 101 post, so we'll keep it simple. If you follow the two basic guidelines below, ...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...between cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  17. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...suppressor screens These types of screens are very similar to forward and reverse screens, except that they... which are worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes...temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections...

  18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...on many other labs that may be doing something similar. There may be other variants of this that are better...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

  20. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...
Showing: 181 - 200 of 660 results