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Showing: 1 - 20 of 41 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser... TAG Simon Elsaesser 140013 pAS_4xMx PylT C41CA_FLAG_Mx PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser...TAG Simon Elsaesser 140023 pAS_4xMma PylT_FLAG-Mma PylRS AF PylRS AF M. mazei Mammalian TAG Simon Elsaesser...Mammalian TAG Simon Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser ... TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser...Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG Simon Elsaesser...

  2. Cre-lox system

    Type
    Collection
    ...Khakh 105869 pEMS1925 iCre-ERT2 Simpson 105870 pEMS1725 iCre-ERT2 Simpson 106368 pCMV-Tag2B-NCre N-terminal... iCre with MCS for inserting promoter none AAV Simpson 49455 pSH62-EBD Cre-EBD - Estradiol-dependent control...with MCS for inserting promoter, WPRE none AAV Simpson 72255 pCDH-CB-iCre-P2A-tdTomato-T2A-Puro iCre and...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP and pSico PGK puro Lentiviral...off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro... to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers 15912 CTV Cre activates...

  3. CRISPR Guide

    Type
    Collection
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

  4. Antibody Guide

    Type
    Collection
    ...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...62201 Plant yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian... natural type II-A CRISPR arrays capable of simultaneously repressing multiple target genes in Escherichia...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...sequences can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1 and Vsx2 enhancers in the ...

  8. Mammalian RNAi Tools

    Type
    Collection
    ...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Allows for visualization of multiple proteins simultaneously with dual labeling when used with two substrates...by ACP Synthase, thereby permitting selective simultaneous labeling in a sample. Please see the note below...

  10. TALEN Guide

    Type
    Collection
    ...target sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...

  11. Viral Vectors

    Type
    Collection
    ...delivered to a host and generate virus (right), similar to how natural viruses can be delivered to a host...Viruses used to direct these alternate functions simply have to harbor alternate viral genomes, such that...

  12. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...

  13. CRISPR References and Information

    Type
    Collection
    ...gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse, rat...for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...based on shared characteristics and evolutionary similarity. These are grouped into two large classes based...conditions. Genome editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1...

  17. Bacterial Expression Systems

    Type
    Collection
    ...proper conditions and, epitope tags can be used to simplify the purification process. Using plasmids in this...arabinose binding protein (AraF) in the plasmid. Other similar reporters for detecting maltose can be found here...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858...leukocyte protein of 76kDa) SLP-76, SLP76 LOC652578 similar to Fc fragment of IgG, low affinity IIIa, receptor...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ...-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary...pcDNA4/TO/GFP-GIGYF2 GIGYF2 GFP CMV Parkinson's Simon Bekker-Jensen 141323 pENTR-TARDBP-G287A TARDBP ALS...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Zap) New alternately colored FRET sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular ...detect subthreshold membrane potential changes Simultaneous two-photon imaging of action potentials and ...
Showing: 1 - 20 of 41 results