... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...specific recombination sites (in this regard, not so dissimilar from restriction enzyme cloning). To do this ...selection cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and...benefit of Gibson cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen...Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of ...the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently fuse two...

...clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and this process of... vary broadly in their functionality. In their simplest form, plasmids require a bacterial origin of replication... DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene...so they can be distinguished from one another. Similar to DNA replication, the Sanger sequencing reaction...

...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

...infectious transgenic lentivirus is outlined in the simple schematic to the right. 3-4 plasmids are transfected...recommended that you use lentiviral transfer plasmids for simple transfections. Is it feasible to express cDNA from...Often times it is less time consuming and easier to simply add restriction sites onto the insert of interest...

...muscarinic-receptor based DREADDs. Compound 21 has similar potency as CNO, while DCZ is more potent than either...Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons A, Nonneman RJ, Huang X-P, Hufeisen SJ, Guettier...opens in a new window) Slaviero AN, Gorantla N, Simkins J, Crespo EL, Ikefuama EC, Tree MO, Prakash M, ...

...genes that contribute to a phenotype. At their simplest level, pooled libraries are single preparations...parallel sequencing of millions of DNA fragments simultaneously in a single reaction. As discussed above, access...

...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

...for transcription initiation. The -35 position, simply titled the -35 element, typically consists of the...sequences. Operons Although bacterial transcription is simpler than eukaryotic transcription bacteria still have...

...help. Another virus, such as adenovirus or herpes simplex virus, or a genotoxic agent such as UV radiation...

...and light oxygen voltage (LOV) domains function similarly to cryptochromes with light-induced changes in...