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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 424 results for: SUA

Showing: 181 - 200 of 424 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Selecting for seeds that have the tDNA insertions (usually via seed growth on antibiotic-containing media)...from the Dinneny lab that additionally allow for visual selection of transformants based on expression ...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, ...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...launched the Addgene YouTube channel to add a strong visual component to our educational resources. From 2013...a plasmid and its parts that helps the viewer visualize something that cannot be seen with the naked eye...

  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... where cover letters are common, the answer is usually yes! A good cover letter won’t hurt your application... matches the job requirements, and address any unusual circumstances or proactively answer questions the...

  7. How to Be an Excellent Trainee

    Type
    Blog Post
    ...bench mentor is also the scientific mentor…but usually the bench mentor is a staff scientist, postdoc,...the next day. In the latter case, you can say “I usually need time to gather my thoughts, but always have...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...request a DNA pooled library from Addgene, you’ll usually receive an aliquot from the original library constructed...representative NGS results (read counts or another visualization) that confirm diversity of the library sample...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid can more effectively... that lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Pinterest is also a favorite for millions and their visual format is quite appealing to many audiences. While... of these platforms (such as social networking, visual appeal, instant sharing across multiple other sites...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...imminently. Consulting firms deploy case teams, usually with 3-6 consultants, who work on analyzing the...consulting meant for PhDs? Healthcare consultants are usually trained in the life sciences, in business, in healthcare...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ...protein or disruption of a gene, can be easily visualized in a living organism from embryonic development...adulthood. This ability to genetically manipulate and visualize cells and tissues allows scientists to conduct...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...single targeting strand, with the extra strand usually being degraded. The target strand binds to the ...create stable knockdown cells lines, and you can usually find validated shRNA sequences for your intended...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...multimers, since restriction sites in multimers usually repeat in tandem along with the repeated plasmid...multimer in your sample remains unclear after visualizing it on a gel, one of the other methods below should...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...fragments by utilizing regions of sequence homology (usually 30-40bp overlaps). To expand the capabilities of...lab yeast strains since they are prototrophic, usually have low levels of homologous recombination, and... challenging. Small molecule kinase inhibitors usually target soluble kinase domains, but these domains...

  19. Plan Your Experiment

    Type
    Collection
    ... restriction enzymes for further customization Usually paired with a sequencing method Can’t provide specific...
Showing: 181 - 200 of 424 results