...Selecting for seeds that have the tDNA insertions (usually via seed growth on antibiotic-containing media)...from the Dinneny lab that additionally allow for visual selection of transformants based on expression ...

...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...

...immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue...your sample type. Starting with a protocol that usually works in your system and then optimizing from there...

... into a completely new realm. It allows for the usual office dynamics to be mixed up a bit. Who know that...word of mouth - be sure to bring up your event in casual conversation to get people excited!    Laura ...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, ...

...launched the Addgene YouTube channel to add a strong visual component to our educational resources. From 2013...a plasmid and its parts that helps the viewer visualize something that cannot be seen with the naked eye...

... where cover letters are common, the answer is usually yes! A good cover letter won’t hurt your application... matches the job requirements, and address any unusual circumstances or proactively answer questions the...

...cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid can more effectively... that lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy...

... includes visual representations of all detected edit types in the sample. The visualization helps the...

...bench mentor is also the scientific mentor…but usually the bench mentor is a staff scientist, postdoc,...the next day. In the latter case, you can say “I usually need time to gather my thoughts, but always have...

...request a DNA pooled library from Addgene, you’ll usually receive an aliquot from the original library constructed...representative NGS results (read counts or another visualization) that confirm diversity of the library sample...

...Pinterest is also a favorite for millions and their visual format is quite appealing to many audiences. While... of these platforms (such as social networking, visual appeal, instant sharing across multiple other sites...

...imminently. Consulting firms deploy case teams, usually with 3-6 consultants, who work on analyzing the...consulting meant for PhDs? Healthcare consultants are usually trained in the life sciences, in business, in healthcare...

...cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing a homologous... now also takes into account that HDR is rare, usually 2-5% of the edited cells in our hands, these combined...

...protein or disruption of a gene, can be easily visualized in a living organism from embryonic development...adulthood. This ability to genetically manipulate and visualize cells and tissues allows scientists to conduct...

... of being able to see your protein of interest usually outweigh potential negative consequences of the...Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in...

...multimers, since restriction sites in multimers usually repeat in tandem along with the repeated plasmid...multimer in your sample remains unclear after visualizing it on a gel, one of the other methods below should...

...fragments by utilizing regions of sequence homology (usually 30-40bp overlaps). To expand the capabilities of...lab yeast strains since they are prototrophic, usually have low levels of homologous recombination, and... challenging. Small molecule kinase inhibitors usually target soluble kinase domains, but these domains...

...the key to a great image is contrast. The human visual system relies primarily on contrast to identify... is collected and focused onto a camera for visualization. Illuminating a sample in the opposite direction...

... its way toward the specimen. It allows the visualization of fluorescent proteins expressed in the specimen...ability to track immune cells within organs and visualize how cells interact after a particular stimulus...