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We narrowed to 472 results for: Sele

Showing: 181 - 200 of 472 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...chloramphenicol resistance cassette for positive selection and a p15A origin of replication, making it incompatible...Your strain may require additional antibiotic selection or a special growth media, or if your plasmid ...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Thermo Fisher Scientific's Biotinylation Reagent Selection Tool. AviTag and BirA Proteins can also be biotinylated...Photobiotinylation Photobiotin can be used to non-selectively label DNA and RNA. Photobiotin acetate consists...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... into the final translated products. Negative selection markers built into all plasmids in the kit help...studies monitoring sequence dynamics under various selective pressures: Miller, Corwin, et al. "Adaptation...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...necessary to set up a sequence of gates to be able to select and precisely measure your cells of interest. In...                                                                                                        B.      Figure 5: (A) A gate to select the negative live population from my live-dead ...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...intended edit is the biggest determinant when selecting a type of donor. ssDNA templates (referred to ...large insertions such as fluorescent proteins or selection cassettes. This class of template should have ...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...a unique and powerful high-throughput screen to select variants based on performance metrics specifically... was identified from a high-throughput screen, selected for increased voltage response, brightness, and...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes... infected with 96 h lentiviral supernatant and selected with puromycin. Puromycin-resistant cells were...

  8. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...that there is no universal scoring system for selecting a gRNA, as the method of producing the guide (...phenotype is due to an on-target effect. Conclusions Selection of gRNAs for an experiment needs to balance maximizing...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...mouse embryonic stem cell (ESC) line, enrich and select for cells that had successfully incorporated the...eggs, circumventing the need for enrichment and selection offered by mouse ESCs. For example, we often screen...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...identify which insertions were positive or negative selected for during the experiment (Sandoval-Villegas et...and host range are important to consider when selecting a transposon for a mutagenesis screen. For example...

  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...effective and accurate way possible it’s important to select the right test for the job (and, uh, gentle reminder...run it.) You don’t need to be a mathematician to select the right statistical test, just do your homework...

  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...skills who fulfill a long-term function. When a select group of people are responsible for a group of ...of criticism or judgment - nobody likes to feel useless, and excess people who are not contributing will...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...experiments, care still needs to be taken when selecting which particular AAVs to use for your research...’s value greatly. Applying this mantra to AAV selection, there are three important factors related to ...

  16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...broken down broadly into three main steps: 1) Selection and expression of your opsin of choice in the ...features of each of these steps. Today we’ll discuss selection and expression of your opsin, but keep you eyes...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have..., with a choice of either hygromycin or Basta selection. Yang Lab Single Transcript Multiplexing Plasmids...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ...geNorm study (Vandesompele, 2002) will help you select the appropriate reference genes - and it is well...26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation. Biotechniques. 2000 Aug;...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...the cell’s chromosomes. With the addition of a selectable marker, this system allows for the oriP/EBNA1...plasmid can hang around for awhile, even after selection is removed. Yu et al found that ⅔ of subclones...
Showing: 181 - 200 of 472 results