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...Experiment Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery...expression vectors . Select Your Expression and Delivery System Once you have selected your target and designed... to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering... CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will... in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted The exact region...of your protein expressed in your system. HDR : Select a cut site as close as possible to the location...

... plasmid with puromycin selection. See plasmid 24150 for hygromycin selection. Bob Weinberg 10878 pLKO... of shRNA with puromycin selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578...expression of shRNA with neomycin selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Christophe Benoist and Diane Mathis 21915 Tet-pLKO-puro...shRNAs or miRNAs, and use a wide variety of drug selection markers. See Campeau et al. (2009) for additional... CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd Tet-based ... gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006) for additional variants...

...Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top RNA Editing ID Plasmid Gene/Insert Selectable Marker...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit...Return to top Epigenetics ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere...top Prime Edit ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID...

... you can find or select for only the cells that received the plasmid. Selectable Marker...plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...function, selectable markers, and more. Plasmid...cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently used in molecular...many elements to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most...Genome Modification, mRNA Regulation, and More Selectable Markers Species-Specific Expression If you want...

...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ...top Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID ...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid Gene...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable...to top Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ...

... of shRNA with puromycin selection. See Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain...puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection. TetR ...vector with blasticidin selection. See Plasmid #26730 for hygromycin selection. rtTA3 Eric Campeau 128061...article ( Kowarz et al., 2015 ) for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930...expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521 pCW57.1...vector for repressible expression with blasticidin selection. *Note: this vector contains an insert (MAT2A)...for CMV-driven rtTA with EGFP and Blasticidin selection rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV...

...Promoter Selectable Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find more CRISPR...

...Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Selectable Marker PI...Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Plant". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit...

...Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Yeast". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit...top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid... Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid...

...nuclei, AF488, AF555, and AF647 Antibody Selection Selecting the right antibody for your experiment is...section to help you select antibodies for your approach. Primary antibodies To select an antibody, first...Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents are molecular tools...mid-forties. When designing a multiplex assay, selection of the fluorophores used is important. It is best...appropriate lasers and filters for each fluorophore you select. Suggested panels for small multiplex experiments... you may either purchase several to compare, or select one based on price, shipping availability, ease... called secondaries, are usually much easier to select. There are three main considerations. What is the...

...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter...

...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable...Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find more CRISPR...

...This tool can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale...text file you upload. Currently supports a wide selection of organisms. From the Database Center for Life...sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and rat reference...PDF, 364 KB Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9...Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); ...

...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ... to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID ...

...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ... to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top C. elegans...

...gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return to top gRNA-only...gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return to top Multiplex gRNA...gRNA Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return to top Multiplex...for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids and resources. Find more...

...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR...Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...

...Consortium (SGC) collection page contains an extensive selection of plasmids and tags for protein purification.... and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins...Check out our Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors...Inducible Expression Collection for an extensive selection of plasmids using tetracycline-regulated transcriptional.... Check our Cre-Lox Plasmids Collection for a selection of plasmids containing recombinases to induce ...

...to Addgene's Plant CRISPR Resources for a full selection of plasmids expressing different functional variants...Resources Check our Plant CRISPR Resources for a selection of plasmids expressing different functional variants...targeted gene mutation. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation...Reporter Plasmids The table below highlights a selection of popular reporter plasmids that feature fluorescent...

...(eg GFP, Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved... repair) donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas... the Cas9-programming sgRNA and HR donors for selectable genome modification. This system can be used ...