Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 75 results for: Sele

Showing: 1 - 20 of 75 results

  1. Plan Your Experiment

    Type
    Collection
    ...Experiment Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery...expression vectors . Select Your Expression and Delivery System Once you have selected your target and designed... to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering... CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will... in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted The exact region...of your protein expressed in your system. HDR : Select a cut site as close as possible to the location...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids ...directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted...heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... you can find or select for only the cells that received the plasmid. Selectable Marker...plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...function, selectable markers, and more. Plasmid...cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently used in molecular...many elements to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most...Genome Modification, mRNA Regulation, and More Selectable Markers Species-Specific Expression If you want...

  4. CRISPR Plasmids - Plants

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression... efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  5. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ... of shRNA with puromycin selection. See Plasmid #21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain...puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection. TetR ...vector with blasticidin selection. See Plasmid #26730 for hygromycin selection. rtTA3 Eric Campeau 128061...article ( Kowarz et al., 2015 ) for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930...expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521 pCW57.1...vector for repressible expression with blasticidin selection. *Note: this vector contains an insert (MAT2A)...for CMV-driven rtTA with EGFP and Blasticidin selection rtTA-Advanced Mikhail Alexeyev 17492 pLenti CMV...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase.... ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  8. Antibody Guide

    Type
    Collection
    ...nuclei, AF488, AF555, and AF647 Antibody Selection Selecting the right antibody for your experiment is...section to help you select antibodies for your approach. Primary antibodies To select an antibody, first...Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents are molecular tools...mid-forties. When designing a multiplex assay, selection of the fluorophores used is important. It is best...appropriate lasers and filters for each fluorophore you select. Suggested panels for small multiplex experiments... you may either purchase several to compare, or select one based on price, shipping availability, ease... called secondaries, are usually much easier to select. There are three main considerations. What is the...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...

  10. Lentivirus Plasmids

    Type
    Collection
    ...expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible...shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

  11. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  12. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  13. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... and SpCas9, but does not contain markers for selection 1 . Other constructs may be utilized, such as ...ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs in which...Screening CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a...conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...onto LB agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject...initial screen ensures that the correct clone was selected and reproducibility of results. To evaluate the...

  14. CRISPR References and Information

    Type
    Collection
    ...This tool can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale...text file you upload. Currently supports a wide selection of organisms. From the Database Center for Life...sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and rat reference...PDF, 364 KB Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9...Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection marker); ...

  15. Bacterial Expression Systems

    Type
    Collection
    ...Consortium (SGC) collection page contains an extensive selection of plasmids and tags for protein purification.... and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins...Check out our Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors...Inducible Expression Collection for an extensive selection of plasmids using tetracycline-regulated transcriptional.... Check our Cre-Lox Plasmids Collection for a selection of plasmids containing recombinases to induce ...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ...(eg GFP, Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved... repair) donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas... the Cas9-programming sgRNA and HR donors for selectable genome modification. This system can be used ...

  17. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...

  18. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ID Plasmid Gene...Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  19. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids... and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene...gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae...
Showing: 1 - 20 of 75 results