We narrowed to 333 results for: cki

1. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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   Blog Post

   ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

2. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search...

3. Fluorescent Proteins 101: Fluorescent Biosensors

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   Blog Post

   ...extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end ...

4. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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   Blog Post

   ... writing group I am part of is responsible for checking the scientific accuracy of everything that goes...

5. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   Blog Post

   ...individual screens curated for the publication. Clicking on the screen name takes users to a display page...

6. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   Blog Post

   ... days talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene...

7. Choosing Your Perfect Empty Backbone

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   Blog Post

   ...to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g...

8. Choosing a Good Mentor for Scientists

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   Blog Post

   ...advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs...

9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   Blog Post

   ...injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive...

10. Meet the New Addgene Board Members!

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    Blog Post

    ...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...

11. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    Blog Post

    ...Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive...

12. Antibodies 101: Epitope Availability

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    Blog Post

    ...instead of when it is attached to a cell membrane.  Picking the Right Antibody  It can get even more complex...

13. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

    ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely to carry...

14. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post

    ...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...

15. Antibodies 101: Epitope Tags

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    Blog Post

    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...

16. New and Upcoming Viral Vectors - December 2019

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    Blog Post

    ...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as...

17. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    ...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ...

18. Don’t Wait To Launch Your Health Science Startup

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    Blog Post

    ... your entire career in science, you are likely lacking the necessary business background and/or connections...

19. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post

    ...we make, my job choices were mostly by chance. Luckily, around the time I decided to look for work outside...

20. Hot Plasmids: FRET-Based Biosensors

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    Blog Post

    ...firing in neurons and high resolution functional tracking of T lymphocytes, too, making them a versatile...