Skip to main content
Addgene
Showing: 1 - 20 of 31 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ...mRFP1 David Liu 140448 QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg...Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim 177180 pDAS12069_...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice ...applications. Full references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent... for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224 : sgRNAs...10.1038/nbt.2647. Epub 2013 Jul 21. PubMed . Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing...plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Platt...

  3. Bacterial Expression Systems

    Type
    Collection
    ... Genome Engineering Although overexpressing or knocking down a gene can tell you much about its function...crystallography, you’ll likely need to purify it. Luckily, many proteins can be functionally expressed in...Growth of strain USO hisB- pyrF- rpoZ- on plates lacking histidine and containing 3-AT Scot Wolfe Bacterial...- pyrF- rpoZ- , this promotes growth on plates lacking His and containing 3-AT. FLIParaF.Ec-250n 18855...

  4. CRISPR Guide

    Type
    Collection
    ...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...

  5. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

  6. Lentivirus Plasmids

    Type
    Collection
    ... ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 ...inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf...Glutamate iGluSnFR glutamate sensor with improved trafficking to the plasma membrane Acute disruption of the...

  10. Institute for Protein Innovation

    Type
    Collection
    ...ligand-binding pocket Bind specific subunit pairs, blocking ligand binding and receptor function Figure courtesy...

  11. FlyCRISPR

    Type
    Collection
    ...replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. (...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Richard Kriwacki 205856 CL20_mEGFP_MFF_ERBB4 ERBB4 GFP CMV ALS Richard Kriwacki 205928 CL20_mEGFP_SSBP2...Richard Kriwacki 205953 CL20_mEGFP_WASL_VPS13D VPS13D GFP CMV Spinocerebellar Ataxia Richard Kriwacki 206069...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  14. CRISPR Plasmids - Yeast

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  16. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  19. CRISPR Plasmids - Plants

    Type
    Collection
    ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  20. Open Enzyme Collection

    Type
    Collection
    ...Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT Thermus thermophilus...
Showing: 1 - 20 of 31 results