We narrowed to 310 results for: construct

1. DAP arrays for multiplex gene editing

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   Blog Post

   ...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...

2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ...Sequence schematics for PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… Figure 4....

3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...with each cell receiving only 1 TF expression construct. Cells were cultured following a differentiation...

4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   ...an individual effort and more of a collective construction. In helping today and be helped tomorrow. Our...

5. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   ... voltage changes in worms and have made these constructs available at Addgene. These include Arch(D95N...

6. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ... methods have also been patented. In order to construct our color changing flower we are carefully making...

7. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   ...means the mentee needs to be open to change and constructive suggestions and feedback. The best thing to ...

8. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ... us to image R bodies inside cells, and these constructs may be useful to future scientists who would ...

9. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...lentiviruses). Separating Cas9 and the gRNA into two constructs (i.e., using a dual-vector system as opposed ...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...multiple DNA fragments. This system has been used to construct plasmids for use in model organisms such as E....

11. Hot Plasmids - May 2022

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    Blog Post

    ...sinus vein injection of a CAG-driven mNeonGreen construct in mouse. Image from Goertsen D, et al., 2022....

12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...

13. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post

    ...license. Using OCaMP in your lab Several OCaMP constructs are now available through Addgene. Whether you're...

14. Fluorescent Proteins 101: Luciferases

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    Blog Post

    ... vectors, and literally thousands of reporter constructs with a gene of interest already inserted. In ...

15. The time and cost required to make a plasmid

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    Blog Post

    ...Instead of making one plasmid at a time, try constructing several plasmids in parallel. That way, you ...

16. Plasmids 101: The protein expression toolbox

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    Blog Post

    ... promoter or tag prior to introduction of the construct into cells. However, if you would like tunable...

17. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ...of building synthetic genomes or genome-scale construction projects. Not from the ground up, but so that...

18. CRISPR References and Information

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    Collection

    ... Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection and ...triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal...

19. CRISPR/Cas9 FAQs Answered!

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    Blog Post

    ...the upper is because the top figure shows the construct from our 'Split RNA' design, where the pre-processing... lower figure with PX330 and PX335, where the construct is from our 'Chimeric RNA' design (meaning the...

20. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability measurements...