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We narrowed to 8 results for: construct

Showing: 1 - 8 of 8 results

  1. Adenovirus Guide

    Type
    Guide
    ...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency....

  2. Chemogenetics Guide

    Type
    Guide
    ...neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-type specific promoters...common promoters found in chemogenetic receptor constructs and the cell types in which they drive expression...

  3. Promoters

    Type
    Guide
    ...the RNA polymerase. A transcription complex is constructed from the RNA polymerase and several transcription...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...

  7. Molecular Biology Reference

    Type
    Guide
    ...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...

  8. CRISPR Guide

    Type
    Guide
    ... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...
Showing: 1 - 8 of 8 results