We narrowed to 8 results for: construct

... for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high...a growing number of libraries containing pre-constructed standardized modules. In the first assembly step...vectors permits the creation of even more complex constructs constrained only by the ability of E. coli to...standardized parts to create eukaryotic multigene constructs. MoClo Plant Parts Kit Plant Expression Nicola... for hierarchical assembly of plant multigene constructs. MoClo Plant Parts III: Transformation & Genome...containing several cassettes and generate multi-construct transgenic plants. GreenGate 2.0 Toolkit Plant... (Lohmann) to create plasmids with multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit...

...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency....

...neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-type specific promoters...common promoters found in chemogenetic receptor constructs and the cell types in which they drive expression...

...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...

...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...

...interested in. Self-replicate — Once you have constructed a plasmid, you can easily make an endless number...

...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...

... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...