...Gradinaru 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato AAV construct expressing dTomato driven by SST interneuron-targeting...Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213940-PHPeB pAAV_BiPVe3_dTomato_nlsdTomato AAV construct expressing dTomato driven by PV+ basket cell-targeting...Gradinaru 213937-PHPeB pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213936-PHPeB pAAV_BiPVe4_dTomato_nlsdTomato AAV construct expressing dTomato driven by chandelier cell-targeting... 213915-PHPeB pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...

...interactions and a series of FRET standards . Pre-constructed fluorescent biosensors targeting small molecules...also available. Custom biosensors can also be constructed using the cpFRET kit from the Pertz laboratory...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...via a 10 amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Amber...linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus VCA...VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV Heterotrimeric...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct...

...switch CMV Mammalian Livet loxP (floxed) Constructs These constructs allow for Cre-regulated gene expression...Depending on the construct, Cre may activate or repress gene expression. Common construct types include:... can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the shRNA ...lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two...Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References...recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present...expression of your gene of interest. Many Cre constructs also contain fluorescent labels that indicate...

...Worm Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode...characterized which allow researchers to express desired constructs in precise cell types and/or developmental timeframes...plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful...elegans . Synthetic Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology...

...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems. ...and Genomic Tools Reporters and Tags Library Construction Integration Recombination Shuttle Vectors Plasmids...

...of each CRISPR/Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells...does not contain markers for selection 1 . Other constructs may be utilized, such as pX458 (Addgene plasmid...puromycin as selectable markers, respectively, or constructs in which multiple sgRNAs may be expressed from...yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE...conditions for each cell line with a reporter construct to ensure robust plasmid delivery before attempting... that received high levels of the CRISPR/Cas9 constructs. Plate sorted cells individually into 96-well...

...including mutant, knockdown, and overexpression constructs. Each collection page listed below organizes ...Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene expression vectors for in...

...vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann...containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts... for hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular Cloning...

...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible...addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive...transcription Pete Stecha Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...in plants Daniel Voytas Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...

... our inventory of chemogenetic constructs, including DREADD constructs....

...stranded break constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids ...

...small, repeat-laden DNA regions into a single construct would be technically challenging. Thanks to efforts... of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian...6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating...

...assay, and easy to purify using histidine-tagged constructs and routine protein and molecular biology techniques... window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE...

...documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit...Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression...Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto...

...for additional constructs TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses ...Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert and induce your ...

... locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus...pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short...method to tag endogenous proteins by using donor constructs that harbor synthetic short homology arms. Notably...

...complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression...MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy...

...expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's lab. Neutralizing...

...Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized...

...Davidson lab fluorescent protein fusion constructs and backbones....