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We narrowed to 371 results for: fus

Showing: 181 - 200 of 371 results
  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... 18;127(7):839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene ...Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco...
  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of...antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and...
  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ...triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright targeted...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor...
  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria for antibiotic resistance... treatment. This is called a Kirby-Bauer disk diffusion test or Kirby-Bauer susceptibility test (Figure...
  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...
  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...
  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...
  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...
  9. CRISPR Cheat Sheet

    Type
    Blog Post
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...
  10. Chemogenetics AAV Preps

    Type
    Collection
    ...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...mCherry fusion none 5 Bryan Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50455...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50458...-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry fusion Cre-dependent 8, rg* Bryan Roth 50474 pAAV-hSyn-hM3D...
Showing: 181 - 200 of 371 results