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We narrowed to 63 results for: fus

Showing: 1 - 20 of 63 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yardena Samuels 29591 426Gal-FUS FUS GAL1 ALS Aaron Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler...416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS Aaron Gitler 29596 426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS...29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29598 426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS...29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29600 426Gal-FUS-1-453aa-YFP FUS YFP GAL1 ALS...29601 426Gal-FUS-1-501aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29603 426Gal-FUS-101-526aa-YFP FUS YFP GAL1 ALS...29605 426Gal-FUS-267-526aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29606 426Gal-FUS-285-526aa-YFP FUS YFP GAL1 ...

  3. TREAT-AD Plasmid Collection

    Type
    Collection
    ...Structural Genomics Consortium (SGC) Plasmids Haian Fu Lab plasmids Opher Gileadi Lab plasmids (Link opens...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...one of the following genes: SOD1, C9ORF72, TARDBP, FUS and OPTN. Huntington's Disease Huntington’s disease...Study intrinsically disordered protein regions with FUS and HNRNPA1 plasmids . (Shin et al. Cell. 2017) Study...

  6. COVID-19 Resources

    Type
    Collection
    ... K., Miao, J.L., Cui, H.Y., Huang, M., Zhang, J., Fu, L., Yang, X.M., Zhao, Z., Sun, S., Gu, H., Wang,...coronavirus class I fusion protein upstream of rather than adjacent to the fusion peptide . Journal of...SARS-CoV Spike protein, activating its membrane fusion function (Bosch et al., 2008). TACE plasmids - ...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014....convert adenosine to guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine...modifications at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors can ...transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions...

  8. CRISPR Guide

    Type
    Collection
    ...Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung...Transcriptase (M-MLV RT) is fused to the C-terminus of Cas9 H840A nickase. The fusion enzyme can catalyze targeted...dCas9-VPR — dCas9 fused to several different activation domains in series; a single fusion protein, instead...constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects of CRISPRi/a may...Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources Plan Your Experiment...This means proteins with other functions can be fused to a dCas9 and delivered to specific genomic locations...

  9. Chemogenetics AAV Preps

    Type
    Collection
    ...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...mCherry fusion none 5 Bryan Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50455...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50458...-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry fusion Cre-dependent 8, rg* Bryan Roth 50474 pAAV-hSyn-hM3D...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...collection page Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further... and of the fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein...MBP-TEV N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in ...vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more...insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible... by: Species-Specific Expression Epitope Tag or Fusion Protein Function: Viral Vector Delivery, Genome... vectors - Inducible expression, fusion proteins, and more pGBKT7-GW - Gateway...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...LumiScarlet : A fusion of LumiLuc (ATP-independent luciferase derived from teLuc) fused to mScarlet-I. ..., Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale...pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-...

  12. Promega Plasmid Collection

    Type
    Collection
    ...vectors for sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag... sensitive gene expression analysis and protein fusion vectors for detecting and tracking proteins using...high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link opens in a new window) is a 19.1 ...detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular and extracellular...protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in a new window) technology...puts control of the function and properties of a fusion protein in your hands, thanks to interchangeable...PPI) studies, where each protein of interest is fused to either LgBiT or SmBiT and expressed in live cells...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...example, if the marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...has assembled a collection of popular fluorescent fusion proteins that are targeted to various organelles...well-characterized marker's localization pattern, you should fuse your protein of interest with a fluorescent protein...Primary cilia (Neurons) Drd1 EGFP Kirk Mykytyn *Fusions to other FPs can be found by searching the complete...microtubule-binding domain mCherry Maria Harrison *Fusions to other FPs can be found by searching the complete...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...for expression as TALE-LSD1 fusions. It was recently shown that TALE fusions with the histone demethylase...are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors...Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter... Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...Visualization [TGV]). pTALYM3 and pTALYM4 contain TALE fused with mClover and mRuby2, respectively, and can be...

  15. CRISPR Plasmids and Resources

    Type
    Collection
    ... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given...

  16. Bacterial Expression Systems

    Type
    Collection
    ...tags so you can express your protein of interest fused to FPs and monitor their localization in live cells...Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable...test if two proteins bind or interact together by fusing each of them to two FPs whose emission and excitation...exciting the donor. In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins...plasmids containing FPs and split FPs that can be fused to your proteins of interest in bacterial FRET and... Bacterial Two-Hybrid (B2H) System allows you to fuse proteins of interest to transcriptional regulatory...01 Promoter activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength...

  17. Fluorescent Proteins: FRET

    Type
    Collection
    ...proteins fused with linkers of varying lengths. Description Article ECFP-EYFP FRET standards, fusion proteins...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred to...a protein where the donor and acceptor are both fused to the same protein (also referred to as intramolecular...mTurquoise2-mVenus FRET calibration standards and fusion proteins Feldmann et al. (2023) Protocol for deriving...fast-maturing red fluorescent protein Variety of fusion proteins, positive controls for many FRET pairs...

  18. Plan Your Experiment

    Type
    Collection
    ...or Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their...copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair...editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a...permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB...permanently modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64...transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need to be within...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase... from the Michael Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...

  20. Optogenetics AAV Preps

    Type
    Collection
    ...105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Ofer Yizhar...105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn GtACR2 (soma-targeted) FusionRed Cre dependent 1, 5, rg* Ofer...105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed Syn GtACR1 (soma-targeted) FusionRed Cre dependent 5 Ofer Yizhar...
Showing: 1 - 20 of 63 results