...Yardena Samuels 29591 426Gal-FUS FUS GAL1 ALS Aaron Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler...416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS Aaron Gitler 29596 426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS...29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29598 426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS...29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29600 426Gal-FUS-1-453aa-YFP FUS YFP GAL1 ALS...29601 426Gal-FUS-1-501aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29603 426Gal-FUS-101-526aa-YFP FUS YFP GAL1 ALS...29605 426Gal-FUS-267-526aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29606 426Gal-FUS-285-526aa-YFP FUS YFP GAL1 ...

... three different sites in the EGFP reporter gene (Fu et al., Nat Biotechnol. 2013). The expression plasmids...vector ( JDS246 ). Additional details can be found in Fu et al., Nat Biotechnol. 2013 and users are encouraged...

... to modify four different endogenous human genes (Fu et al., Nat Biotechnol. 2013). The expression plasmids...vector ( JDS246 ). Additional details can be found in Fu et al., Nat Biotechnol. 2013 and users are encouraged...

...sites in various endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The expression plasmids...pMLM3613 ). Additional details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are encouraged...

...target sites in endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The Cas9 expression...expression of Cas9 in zebrafish can be found in Hwang & Fu et al., Nat Biotechnol. 2013. The guide RNA ( gRNA...

...Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules...

...Consortium (SGC) Plasmids Opher Gileadi Lab plasmids Haian Fu Lab plasmids The TREAT-AD centers are focused on ...

...modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has ...

...Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell...

...one of the following genes: SOD1, C9ORF72, TARDBP, FUS and OPTN. Huntington's Disease Huntington’s disease...expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17. Next-...

...Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014....convert adenosine to guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine...modifications at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors can ...transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions...

...nuclease specificity using truncated guide RNAs. 2014. Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. Nat Biotechnol...induced by CRISPR-Cas nucleases in human cells. 2013. Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung...also be fused with transcriptional repressors or activators, and targeting these dCas9 fusion proteins...increase HDR frequency. Additionally, Cas9-CtIP , a fusion of Cas9 and CtIP, a protein involved in double-...editors (ABEs) . Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...

...EF1alpha-GFPcre Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha ...Cre-EGFP fusion EF-1 alpha Mammalian Sauer 11956 pBS594 promoterless EGFPcre Cre-EGFP fusion in a promoterless...CRE-GFP fusion none Mammalian Sauer 11960 pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV... CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980...Cre fused to the human Estrogen Binding Domain (EBD) TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to ... mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion hSyn AAV Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion CMV AAV Wilson...

...GFAP nEF E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity...Inhibition HA fusion none 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5 ...mCherry fusion none 5 Roth 50479 pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 5...-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362...-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D...pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth 50458 pAAV-hSyn-DIO-rM3D...

...VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca...Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns) or NanoLuc...pHlash , pRSETb-pHlash : A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through...Firefly/Renilla Creation ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV ...ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2...ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale ...Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale...

...interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest fused to the...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred to...a protein where the donor and acceptor are both fused to the same protein (also referred to as intramolecular...following plasmids can be used to create a fluorescent fusion protein with your gene of interest and the listed...mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean mCerulean...mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean mTurquoise2...

...recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further...the sequence of your gene and of the fusion protein. Tag or Fusion Protein Common uses Representative...vector backbones curated by species, epitope tags/fusion protein, selectable marker, viral expression, and... by: Species-specific expression Epitope tag or fusion protein Selectable markers Viral expression and... vectors - Inducible expression, fusion proteins, and more Sandmeyer Lab...mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning of ...the function of your favorite gene. For example, fusing your protein to an epitope tag, such as Flag...

...between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion protein in pACλCI-β-flap results...interest are fused to AraC or a dominant negative AraC. Preferential association of AraC-fusion homodimers... expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and C-terminal fluorescent...that can be used to create fluorescent protein fusions with your protein of interest. See an overview ... of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid. pZE31...

...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize...

... is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein...examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy...Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various organelles...organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that...Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete...Microtubules ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete...Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete...