...nuclease specificity using truncated guide RNAs. 2014. Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. Nat Biotechnol...induced by CRISPR-Cas nucleases in human cells. 2013. Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung...also be fused with transcriptional repressors or activators, and targeting these dCas9 fusion proteins...increase HDR frequency. Additionally, Cas9-CtIP , a fusion of Cas9 and CtIP, a protein involved in double-...editors (ABEs) . Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...

...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated...-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

...overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ...different molecular biology techniques (such as fusing it with different tags, putting it under a variety...ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase...molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments.... Similar to Gibson, this method can efficiently fuse two (or more) fragments of dsDNA that have 30 or... of DNA that share no end homology can still be fused together in a seamless manner. To accomplish this... (or more) fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base...

...: Tet- or Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids available...system through the addition of a chimeric 5' LTR fused to a heterologous promoter on the transfer plasmid...Wild type Hybrid: 5'LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...

...cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized B cells...fluorophores with minimal signaling overlap to avoid confusing data. Check that your machine has appropriate...