We narrowed to 1,075 results for: ica;
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TypeCollection...Expressions website for additional images, tutorials, optical microscopy protocols and resources. Or read our...
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TALEN Expression Vectors
TypeCollection...methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details... -
MAPK Plasmids
TypeCollection... acting as an integration point for multiple biochemical signals. In collaboration with numerous research... -
Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection...web-based ZiFiT Targeter software to enable identification of potential RGN target sites and to facilitate... -
AAV Viral Preps
TypeCollection...Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable... -
Structural Genomics Consortium Plasmids
TypeCollection...the three dimensional structures of proteins of medical relevance, and place them in the public domain ... -
Plasmid Collections
TypeCollection...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...Viral Vector Resources Addgene's Data Hub Curated technical reports from the research community demonstrating... -
Serotype Testing AAV
TypeCollection...also available as 20 µL. The 20 µL option is an economical choice that can be used to validate the performance... -
CRISPR Plasmids and Resources
TypeCollection...fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries are a powerful tool ... -
Neurodegeneration Plasmid Collection
TypeCollection...Tev CMV ALS Jessica Henty-Ridilla 204223 CMV-Halo-10-PFN1-Y6D PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla...Tev CMV ALS Jessica Henty-Ridilla 204225 CMV-Halo-10-PFN1-G118V PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla... -
Retrovirus Plasmids
TypeCollection...protein. When scientists discuss retrovirus, they typically are referring to a subset of retroviruses called... -
Arf GTPase Family
TypeCollection...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane... -
TALEN Plasmids and Kits
TypeCollection...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...TACGAGGATCTGGTCCACtacggcatggatgcggTCGGGATCCCGTCTCCGA mical2b TAL3492 & TAL3493 TGGGGGAGACGGAGGAAGaacggacatctcaggcCGGTCAGCTTTTTGAGAA... -
Penn Vector Core Partnership with Addgene
TypeCollection...discontinued. The following viral vectors in the indicated serotypes are no longer available as aliquots.... -
A Control for All Seasons
TypeBlog Post...results. Replicates Replicates are another critical control in biological experiments. Replicates can be...inclusion of biological replicates such as multiple mice in each group and C, technical replicates or repeated...meaningful results. Biological replicates are distinct samples that are treated identically, used to assess...recorded would be due to natural biological variation. This information is critical for correctly interpreting...be technical or biological and are included to improve the precision and accuracy and therefore the statistical...statistical power of an experiment. Technical replicates are repeated measurements of the same sample ... rely on the results of a single mouse. Biological replicates help you to determine which responses arise... -
Deep Dive: qPCR
TypeBlog Post... Standard Curve? Amplification Efficacy Calculation Amplification Efficacy Assumptions Standard...increase your understanding and technical proficiency with common applications - now let’s dive right in! ...differences of twofold or greater with good statistical significance (Taylor, 2017). With the right conditions...single peak, indicating one amplicon and no primer dimers. (b) A shoulder peak indicating primer dimers...values within each set of triplicates to understand variation due to technical error. Plate readouts can... be less than 0.1. If you suspect that amplification efficacy differences may be a concern in your reaction...genes can have different efficacies DART-PCR No Raw Data Statistically defined log-linear phase... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...Origin of Replication? See our Plasmids 101: Origin of Replication blog). The replication problem Plasmids...directly inhibit replication by binding to the origin of replication and blocking replication machinery (del... of microbial plasmids: classification and identification based on replication and transfer systems and...plasmid that contains the origin of replication and the replication control machinery (Need a refresher... that inhibits replication when copy number in the cell is high, but allows replication when copy number...either indirectly inhibit replication through inhibiting translation of replication machinery proteins, or...antisense RNAs inhibit plasmid replication. Figure 1: The replication problem. Iterons are repeated...