We narrowed to 131 results for: ica;

...comparison of a genetically encoded sensor and small molecule indicator: implications for quantification of cytosolic...Javier García-Sancho Calcium Genetically encoded calcium indicators for optical imaging (GECO1, GECO1.2),..., blue, and red indicators An Expanded Palette of Genetically Encoded Ca 2+ Indicators. Science. 2011 ... Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator with ...Calcium Near-infrared indicator iGECI A near-infrared genetically encoded calcium indicator for in vivo imaging...KIRIN1) fluorescence indicators of K + Genetically encoded fluorescent indicators for imaging intracellular...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...

...Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods Visualization...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments...this can vary significantly based on the antibody and conjugate chosen. Signal Amplification The amount ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE...concentrates in the sample. Controls for quantification methods Quantification methods should be run with: a positive...

...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. PMID:... organisms, but modifications to various Cas enzymes have extended CRISPR applications to increasingly...takes place during the S and G2 phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating...genes. Each library typically contains ∼3–6 gRNAs per gene to ensure modification of every target gene...following the amplification protocol specified by the depositing lab. After amplification, you’ll need ...heritability. Not all modifications are stable across cell divisions, and some types of modifications are more frequently... subsequently isolated by affinity purification. After purification, molecules associated with the locus...

...Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production...179464-AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ... 113049-AAV9 pAAV-hSyn-GRAB_DA1m express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_...

...controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV...controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV...plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes necessary for the...

...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...to destroy the phages upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based on shared...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that... PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve the tool...various species, as well as the development of new applications. The first CRISPR papers described two main ...

...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable Markers...collection page Zebrafish CMV, h2afv, XlEef1a1 See our dedicated Zebrafish Plasmids and Resources collection page...Additionally, tags are commonly added to aid in protein purification. Just remember to remove the stop codon for ...myristoylation signal GST Protein purification pEBG - N-terminal GST for mammalian...expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial expression with MBP-TEV...MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression vector for His8...

...Catalytically inactive CRISPR plasmids for purification of target loci....conventional methods for genomic purification, CRISPR-based purification methods are more straightforward and...plasmids designed for the purification of genomic loci. To learn more about purification using CRISPR and other...chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular...DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA... can be fused to dCas9 or gRNA for efficient purification. Various epitope tags including 3xFLAG-tag, ...The locus is subsequently isolated by affinity purification against the epitope tag. Compared to conventional...

...Isotype Reactivity Recommended Applications PI Browse Glypican Antibodies Glypicans are a family of highly conserved... includes anti-glypicans that accurately detect glypican presence and explore glypican function, providing...targeting axonal guidance receptors and ligands (Glypicans and Integrins), components in the synapse (Neurexins...monoclonal antibodies with high specificity for critical axon guidance signaling proteins and growth cone...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic...formation, neural connectivity, plasticity and neurological disorders. The initial collection includes antibodies...

...differentiate into multiple cell types, but are typically limited to related cell lineages. Pluripotent ...organism. iPSC technology removes the possible ethical concerns related to embryonic stem cell use and...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming...Blood Cells. Stem Cells. 2012 Nov 29. Yamanaka Replicating EBNA1 episome Human Non-integrating EBNA1-mediated... Nat Methods. 2011 May;8(5):409-12. Yamanaka Replicating EBNA1 episome Human Non-integrating polycistronic... S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-...Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating EBNA1-mediated...

...for protein purification. The pTD Plasmid Series contains plasmids suitable for replication in many gram-negative...expression plasmids that can be used for protein purification, protein visualization, study of protein interactions...Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...bacteria as model systems to answer many interesting biological questions. Escherichia coli is the most widely...expression plasmids to find out more! Protein Purification To study the function, structure, and activity...through affinity chromatography. To facilitate the purification process, proteins are often expressed alongside...

... opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a ...window) PMID: 16615965 Leonard et al. 2017. Clinical and biological progress over 50 years in Rett syndrome...months of age. A defining feature, with onset typically between 18 to 30 months, is regression of previously...of Rett syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of...MECP2 gene. Causative mutations for Rett syndrome typically arise spontaneously, although in rare cases Rett...determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens in a new...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...

...make targeted epigenetic modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic... H3K4 and H3K9 methylation Epigenetic modification is typically targeted to a specific promoter or enhancer...designed for epigenetic modification. To learn more about epigenetic modification with CRISPR and other ...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...enhancer for your gene of interest. These modifications persist over time and are potentially heritable...chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...

... PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter Selectable Marker Publication Return... PI Publication Return to top RNA Editing ID Plasmid Gene/Insert Selectable Marker PI Publication Return...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert... ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Visualize ID Plasmid Gene/Insert...

...the most abundant forms of posttranslational modification in cells and research into its many roles in...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., 2013) and improving...popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus plasmid...subpools of ~11,000 unique phosphosites. Iterative Synthetically Phosphorylated Isomers (iSPI) The iSPI resource...pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: ...an orthogonal translation system with enhanced biological tolerance . Molecular Systems Biology , 19 (8...phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions ....

...Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI Publication Return to top Cut...Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication Return to...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...top Purify ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO...Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding... to top RNA Targeting ID Plasmid Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...cell, and specifically targets the starter cell via the EnvA receptor. RABV is able to replicate in the ...which is not required for the transcription or replication of the genome within infected cells, but is required...present, the virus can spread from this cell to synaptically connected cells (meaning, it can spread to cells... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...complementation, G-deleted rabies virus can spread to monosynaptically connected cells. To further refine the targeting... was modified so that it could only infect a genetically specified neuronal population. This is done using...leukosis virus. EnvA can direct virus infection specifically into cells that express the TVA viral receptor...

...activity in a variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...within 1 business day regarding your account verification. Once your account has been verified, you will.... Pricing Addgene is a nonprofit organization dedicated to providing the scientific community with open...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account...depositing institution(s). It governs the transfer of biological materials and intellectual property rights, and...