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We narrowed to 17 results for: ica;

Showing: 1 - 17 of 17 results

  1. Antibody Guide

    Type
    Guide
    ...Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods Visualization...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments...this can vary significantly based on the antibody and conjugate chosen. Signal Amplification The amount ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE...concentrates in the sample. Controls for quantification methods Quantification methods should be run with: a positive...

  2. CRISPR Guide

    Type
    Guide
    ...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. PMID:... organisms, but modifications to various Cas enzymes have extended CRISPR applications to increasingly...takes place during the S and G2 phases. Chemically or genetically inhibiting genes involved in NHEJ or upregulating...components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. In most applications, a large portion...genes. Each library typically contains ∼3–6 gRNAs per gene to ensure modification of every target gene...following the amplification protocol specified by the depositing lab. After amplification, you’ll need ...heritability. Not all modifications are stable across cell divisions, and some types of modifications are more frequently...

  3. Molecular Biology Reference

    Type
    Guide
    ...enable isolation and purification of cellular components, such as DNA, but practically this isolation is ...fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal ...archaea and eukaryotes, they play the most significant biological role in bacteria where they can be passed... Origin of Replication ( ori ) DNA sequence which directs initiation of plasmid replication (by bacteria...bacteria) by recruiting DNA replication machinery. The ori is critical for the ability of the plasmid to be...generally 1,000-20,000 basepairs) for physical isolation (purification) and manipulation. With current cloning...complementary, strand. Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and ...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...potential formation of replication-competent viruses. While AAV themselves are replication incompetent, there...production of replication-competent viruses that would be able to infect cells and replicate autonomously...organism to target large or diffused biological structures. Specifically, researchers were interested in creating...research, and other biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in... actively causing symptoms or replicating. Lytic cycle Viral replication process where the virus uses ...into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and reproduce.... The Rep region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which...

  5. Chemogenetics Guide

    Type
    Guide
    ...responded to a clinically approved drug, the PSAM domain was engineered to respond specifically to the anti-smoking...coelenterazine (CTZ) or chemical variations thereof (hCTZ), or furimazine (Fz) or chemical variations thereof...integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. Proc...unique and not a common property of either typical or atypical antipsychotic drugs: is M1 agonism a pre-...chemogenetics for research and potential clinical applications. Science. 364(6436):eaav5282. PMID: 30872534...neurons) that can be turned on or off by the application of a small molecule ligand. The ideal chemogenetic...been shown to be involved in a wide variety of biological processes, including initiating signaling pathways...

  6. Optogenetics Guide

    Type
    Guide
    ...proteins; e.g., microbial opsins and optical switches Sensors are genetically-encoded reporters of molecular...Deisseroth K. 2005. Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci...MJ. 2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J...17375185 Kim B, Lin MZ. 2013. Optobiology: optical control of biological processes via protein engineering. Biochem... including explanations of microbial opsin and optical switch terminology. Science...Optogenetics Guide Introduction Microbial Opsins Optical Switches References Introduction The field of optogenetics...their functions into two groups: Actuators are genetically-encoded tools for light-activated control of ...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...vectors are common in clinical applications, like gene therapy, as you can more specifically dictate the target...and tropism. Replication-competent The ability of a virus (or viral vector) to replicate and reproduce...hosts in order to continue their lifecycle and replicate. This feature can be harnessed for stable delivery..., whereas gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses...Lentiviral Vectors The genome of lentiviruses typically ranges from 8–10 kb, encoded on RNA (Figure 1)...of safety improvements and the potential for replication-competent lentivirus production — that is, creation...particles that could infect cells and further replicate on their own. While the viral components are still...

  8. Adenovirus Guide

    Type
    Guide
    ... DNA replication and host cell manipulation. Late transcripts are expressed after DNA replication starts...production of replication-competent adenoviruses (RCAs) that would be able to infect cells and replicate autonomously..., which has limited their use in therapeutic applications. However, this has also made them the most widely...presence of adenoviral genes E1, E4, E2a and VA for replication. For more information about AAVs, read our AAV...be included between the ITRs is limited by the physical space available inside of the adenoviral capsid...whereas the E1 region is essential for viral replication and therefore needs to be supplied through other... other early gene regions, E2 and E4. These modifications aimed to lower immunogenicity issues observed...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...are commonly used in gene therapy applications to more specifically direct delivery of gene therapies....Pule, M. (2022). Efficient clinical-grade gamma-retroviral vector purification by high-speed centrifugation...and tropism. Replication-competent The ability of a virus (or viral vector) to replicate and reproduce...hosts in order to continue their lifecycle and replicate. This feature can be harnessed for stable delivery..., whereas gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses... for safety to reduce the chance of creating replication-competent retroviruses. For a summary of all ...gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression of ...

  10. Plan Your Experiment

    Type
    Guide
    ...Manipulation Select Expression System Design gRNA Publications Get Started CRISPR is a powerful system that...may not be a perfect plasmid for your specific application, and in such cases, it may be necessary to customize...reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional Considerations Knockout ...method Expression System Components of System Application Mammalian expression vector Cas enzyme promoter...EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter gene (e.g. ...gene you target will depend on your specific application. For example: To activate or repress a target...needs to match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ...think of each cell carrying it as being a biological replicate for that plasmid. Library screens can be... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to..., semi-random sequences that can be used for applications like lineage tracing or parsing the effects ...

  12. Addgene's eBook Collection

    Type
    Guide
    ...eBook contains practical introductions and helpful tips for antibody-based applications, from flow to ...2021 | Third Edition About This eBook covers practical experimental advice such as tips for planning ...contains an introduction to each vector and its applications, tips for using vectors in your experiments,...your transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101...

  13. Promoters

    Type
    Guide
    ...100-1000 base pairs long and are adjacent and typically upstream (5’) of the sense or coding strand of...back on itself which helps to explain how many physically distant DNA sequences can affect transcription... -35 position, simply titled the -35 element, typically consists of the sequence TTGACA and this element... operator. Operons are common in prokayotes, specifically bacteria, but have also been discovered in eukaryotes...

  14. Cloning

    Type
    Guide
    ...interest and then insert them into plasmids for replication and protein expression. So how do scientists ...restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested and ligated...is done via a single recombination reaction, drastically simplifying the process and reducing the amount...where the nicks will be repaired by the normal replication process. It is important to note that LIC has...

  15. Science Guides

    Type
    Guide
    ...specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated to a ...Chemogenetics Chemogenetics is a technique that uses genetically engineered receptors to selectively control and...

  16. Sequencing Primers

    Type
    Guide
    ...sequencing primers that Addgene uses for sequence verification of deposited plasmids....used a number of primers for sanger sequence verification of deposited plasmids. Below is a list of commonly...

  17. Educational Resources

    Type
    Guide
    ...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...
Showing: 1 - 17 of 17 results