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We narrowed to 760 results for: mpo

Showing: 181 - 200 of 760 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...rates By Mike LacyGlutamate is one of the most important and abundant neurotransmitters, but it is challenging...targeting Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling pathways during embryonic... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...swapping the homology arms for new targets. And, importantly, they found that moving the RNA untranslated ...fully understand how genes are regulated, it's important to know what proteins interact with each other...

  2. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...useful for genetic engineering approaches. Most importantly for this technology, however, is that the transposase...should be tested in the cell line of interest. Importantly, by varying these ratios, you can either maximize...transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration from...lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific...

  3. Antibodies 101: Validation

    Type
    Blog Post
    ...review why validation is so important. Antibodies are a critical component of the researcher toolkit due...or plan your own validation experiments, it’s important to know what you are aiming to achieve. In general...as whole cell lysate or tissue sections. It is important to confirm that your antibody is not binding to...proteins bound to the target protein, so it’s important to understand if protein:protein interactions ...

  4. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...I have an important question: How much coffee does it take to fuel more than 30,000 neuroscientists over...needed to keep up with all the awesome talks, symposiums, poster sessions, exhibit booths, happy hour ...epileptic seizures in a mouse model. I sat in on a symposium discussing progress made in reprogramming astrocytes...scientific community. All of this speaks to the importance of scientific sharing to the neuroscience community...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...reason it is virtually impossible to determine specific protein turnover or temporal expression using standard...localization, or leading to protein aggregation. Another important parameter to consider is the contrast ratio- a...intensity, possible excitation lasers) it is also important to optimize your activation protocol for your ...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing optical...

  6. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...manager can be efficient without delegating and empowering the team to solve problems together (tactics ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

  7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...projects spanning from the identification of plant compounds and insecticidal proteins to the development of...Improvement of Cowpea, a knowledge database for this important agricultural plant. Her current research focuses... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...

  8. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...as well as the serious business of science. Two symposia on Sunday afternoon were dedicated to science ...Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...downstream control – this is important later! Next, additional components are added - the ‘bells and whistles...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...scientists start out in academia, it is especially important for graduate students and postdocs to get a perspective...interesting topics Having strong non-science skills is important for success Must always justify use of resources...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is...scientists following diverse career paths is an important first step. Developing mentoring relationships...

  11. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...for this purpose. This second area is extremely important and given the lack of attention to it, it is an...tool unique. The CRISPR Software Matchmaker is composed of these features and enables you to select the... interact with the software. This category is important for users wishing to select tools based on their...Matchmaker, it provides a list of terms I think are important to define for the CRISPR software space. It is...many different communities that it is all but impossible to fairly judge individual tools. I believe at...

  12. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...colors of orange and white. The decor was very contemporary and futuristic with block chairs in the hallway...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...for Fragmentation System and this is the most important part of this newly released prep kit. In their...for the research community, so it is extremely important for us as a company to constantly update our processes...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...a PhD student in his first year. “It was very important to me to be with them in the lab when they started...the lab and to take things slowly! It is more important to carefully select first hires who are well-suited...people talk themselves out of great projects as ‘impossible' or useless.” How do you run your lab differently...a challenging process. Each PI emphasizes the importance of slowly building up your lab and selectively...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...Before doing a transfection experiment, it is important to determine the proper concentration required...

  15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...nuclease for your experiments, there are three important factors to consider: site accessibility, specificity...potential for off-target cleavage should be an important consideration during any experiment. Sensitivity...stringent PAM specificity. This can be especially important for base editing techniques, where Cas9 must be...many options! Comic by Maya Kostman. Another important consideration when choosing a Cas9 is balancing...

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

  17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...transcription factor important for T cell differentiation (a cell type important for adaptive immune responses... to generate a transgenic fly There are a few important points to consider when choosing the vector you...affecting the body color of the adult fly). This is important to note so that you know what to look for. Making...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics uses genetically... on and off gene expression while imparting spatiotemporal control, reversibility, and repeatability. .... The system developed by the Sata lab is composed of two fusion proteins: 1) the genomic anchor - an ...) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58...Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR/Cas9 Function in Cells and...

  19. dTAG - You're it!

    Type
    Blog Post
    ...transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...degradation In the Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest... chimeras For each protein-of-interest, it is important to determine which terminus can accommodate the...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...Nature has evolved ways to prevent degradation of important cellular RNA molecules with post translational...Frontiers in Genome Editing). This is particularly important in experiments in which viability is being compromised...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...
Showing: 181 - 200 of 760 results