...screening out irrelevant candidates, and, more importantly, it’ll help you define salary costs. 4. Ascribe...BEFORE searching for a faculty position. This is important to ensure that you’re looking at institutes that...make these decisions in the following order of importance: 1. First make decisions based on experimental...and availability of the equipment is of utmost importance. Conducting a successful lab start up negotiation...resource budget, and know how to decide which components are the most critical to your research, negotiations...

...should I take to get to work?”; we often make important life decisions without much logic - even choosing... of work and communication before making this important decision. I’ll highlight some of these questions... if it is what is best for you. Some of these important questions will be easy to ask the lab head, and...rise of movements such as the Future of Research Symposium and #MeTooSTEM, academic bullies are hard to stop—they...information Overwork – including undue pressure, impossible deadlines, unnecessary disruptions Destabilization...

...rates By Mike LacyGlutamate is one of the most important and abundant neurotransmitters, but it is challenging...targeting Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling pathways during embryonic... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...swapping the homology arms for new targets. And, importantly, they found that moving the RNA untranslated ...fully understand how genes are regulated, it's important to know what proteins interact with each other...

...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...useful for genetic engineering approaches. Most importantly for this technology, however, is that the transposase...should be tested in the cell line of interest. Importantly, by varying these ratios, you can either maximize...transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration from...lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific...

...review why validation is so important. Antibodies are a critical component of the researcher toolkit due...or plan your own validation experiments, it’s important to know what you are aiming to achieve. In general...as whole cell lysate or tissue sections. It is important to confirm that your antibody is not binding to...proteins bound to the target protein, so it’s important to understand if protein:protein interactions ...

...I have an important question: How much coffee does it take to fuel more than 30,000 neuroscientists over...needed to keep up with all the awesome talks, symposiums, poster sessions, exhibit booths, happy hour ...epileptic seizures in a mouse model. I sat in on a symposium discussing progress made in reprogramming astrocytes...scientific community. All of this speaks to the importance of scientific sharing to the neuroscience community...

...reason it is virtually impossible to determine specific protein turnover or temporal expression using standard...localization, or leading to protein aggregation. Another important parameter to consider is the contrast ratio- a...intensity, possible excitation lasers) it is also important to optimize your activation protocol for your ...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing optical...

...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...manager can be efficient without delegating and empowering the team to solve problems together (tactics ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...projects spanning from the identification of plant compounds and insecticidal proteins to the development of...Improvement of Cowpea, a knowledge database for this important agricultural plant. Her current research focuses... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...as well as the serious business of science. Two symposia on Sunday afternoon were dedicated to science ...Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute...

...scientists start out in academia, it is especially important for graduate students and postdocs to get a perspective...interesting topics Having strong non-science skills is important for success Must always justify use of resources...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is...scientists following diverse career paths is an important first step. Developing mentoring relationships...

...downstream control – this is important later! Next, additional components are added - the ‘bells and whistles...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

...for this purpose. This second area is extremely important and given the lack of attention to it, it is an...tool unique. The CRISPR Software Matchmaker is composed of these features and enables you to select the... interact with the software. This category is important for users wishing to select tools based on their...Matchmaker, it provides a list of terms I think are important to define for the CRISPR software space. It is...many different communities that it is all but impossible to fairly judge individual tools. I believe at...

...colors of orange and white. The decor was very contemporary and futuristic with block chairs in the hallway...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...for Fragmentation System and this is the most important part of this newly released prep kit. In their...for the research community, so it is extremely important for us as a company to constantly update our processes...

...a PhD student in his first year. “It was very important to me to be with them in the lab when they started...the lab and to take things slowly! It is more important to carefully select first hires who are well-suited...people talk themselves out of great projects as ‘impossible' or useless.” How do you run your lab differently...a challenging process. Each PI emphasizes the importance of slowly building up your lab and selectively...

...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...Before doing a transfection experiment, it is important to determine the proper concentration required...

...transcription factor important for T cell differentiation (a cell type important for adaptive immune responses... to generate a transgenic fly There are a few important points to consider when choosing the vector you...affecting the body color of the adult fly). This is important to note so that you know what to look for. Making...

...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

...nuclease for your experiments, there are three important factors to consider: site accessibility, specificity...potential for off-target cleavage should be an important consideration during any experiment. Sensitivity...stringent PAM specificity. This can be especially important for base editing techniques, where Cas9 must be...many options! Comic by Maya Kostman. Another important consideration when choosing a Cas9 is balancing...

... optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics uses genetically... on and off gene expression while imparting spatiotemporal control, reversibility, and repeatability. .... The system developed by the Sata lab is composed of two fusion proteins: 1) the genomic anchor - an ...) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58...Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR/Cas9 Function in Cells and...