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... Plasmids Find Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies ...This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute...

...our CRISPR blog posts Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the...

...Addgene Blog Read a blog post on Our Citations Feature Check out this post on Scientific Reproducibility... Many thanks to Daniela Bourges for editing this post and sharing her technical expertise in persistent...

...commonly prepares NGS samples using both pre- and post-amplification DNA and generates PCR sequencing products...plasmids have dropped out, and comparing pre- and post-amplification sequencing results, you should be ...determine the success of your amplification. I hope this post has helped clarify some of the important considerations...

...cloning post with a few quirks specific to the PCR cloning process - please check out that post if you ...same as those discussed in our restriction cloning post. However, even more than with standard restriction...

...This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for...photostability, and so on; see the previous blog post for more discussion of these factors). In addition...proteins, but these are beyond the scope of this post. Are your fluorescent proteins compatible with your...

... downregulated, examine a protein’s stability or post-translational modifications, or study how a target...learn more about Western blotting check out our blog post The Basics of Western Immunoblotting.) For mass ...details of these methods are beyond the scope of this post.  We hope that this blog has provided you with a...Immunofluorescence View all of Addgene's antibody posts  Resources on Addgene.org: Find plasmids ...

...blog Read all of our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...choosing a cloning method Check out all of our blog posts on plasmid cloning Resources on Addgene.org Read...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

...they're useful, so we've updated our cover letter blog post to help you write the best one possible!  Should...hiring managers In preparation for updating this post, I took an informal poll of Addgene hiring managers.... and now... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in...Cover Letter Guide How to Write a Cover Letter Blog Post from Hubspot ...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...

...Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our... This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate...proteins within these compartments undergo significant post-translational modifications including glycosylation... addition of N-glycans [9]. Both of these posttranslational modifications can disrupt FP folding rendering...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

...Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...including protein expression and correct folding, post-translational modification, protein degradation,...cells. If the problem is that your proteins are not post-translationally modified, co-expression of the enzyme...

...answering all submitted questions via blog post, which we will be posting in the next few weeks.)  Finally, Addgenies...

...MattNeuro) December 4, 2020 Here’s how to enter: Post your picture or video for either contest on Twitter...the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will vote on the entries...

...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our ...viral vector services and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  ...

...articles from your favorite bloggers Like a blog post from a certain author and looking for more from ...can read more about the Addgenie behind the blog post and find more of their work. Articles from guest...

...developing GFP-activated proteins, read our recent blog post. Check out the break down below the audio player...Resources on the Addgene Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in...

...The following post was contributed by the Developmental Studies Hybridoma Bank. Antibodies are the most...Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific Sharing: The 10 Most Distributed...

...time based on your cell line and assay: Assay Days post-infection mRNA knockdown (quantitative PCR) ≥3 days...

...This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie...and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’s potential for monogenetic diseases, ...complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use in treating...

...This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal...made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins of the system and ... that have happened since our original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed...