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Showing: 1 - 20 of 22 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (96 h post-transfection): Add valproic... Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4%...168 h post-transfection): Harvest antibody. Section 4: Harvest antibody At 168 hours (1 week) post-transfection...... Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection...valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% BCD Feed...a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM ...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...dilutions of 64108 pHAGE-TO-dCas9-3XmCherry. 72 hours post transduction, cells were assayed for mCherry expression...

  3. General Transfection

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh ...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...tips on AAV titering using ddPCR, read this blog post on ddPCR for AAV quantification. This protocol was... tips on AAV titering using ddPCR, read our blog post....

  5. Lentivirus Production

    Type
    Protocol
    ...(pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace with fresh media...cells. Virus can be harvested at 48, 72, and 96 h post transfection in individual harvests or a combined...

  7. Video Library

    Type
    Protocol
    ...for working with tissue culture in the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...120 h. Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

  9. Immunocytochemistry

    Type
    Protocol
    ...like... Antibody Plasmid Collection Antibody Blog Posts Western Blot Protocol Recombinant Antibody Purification...

  10. Colony Formation Titering Assay

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...

  15. Western Blot

    Type
    Protocol
    ...like... Antibody Plasmid Collection Antibody Blog Posts Immunocytochemistry Protocol Recombinant Antibody...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...Agar Plates You may also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction...

  18. Kit Free RNA Extraction

    Type
    Protocol
    ...For more tips on working with RNA, read this blog post on RNA extraction without a kit. Last Update: April...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure access...
Showing: 1 - 20 of 22 results