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Showing: 1 - 20 of 756 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (96 h post-transfection): Add valproic... Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4%...168 h post-transfection): Harvest antibody. Section 4: Harvest antibody At 168 hours (1 week) post-transfection...... Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection...valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% BCD Feed...a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM ...

  2. Guide RNA Expression Plasmids for EGFP

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently described the use CRISPR-...Nat Biotechnol. 2013 and users are encouraged to post questions to the Genome Engineering newsgroup (which...

  3. Guide RNA Expression Plasmids for Endogenous Human Genes

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently described the use CRISPR-...Nat Biotechnol. 2013 and users are encouraged to post questions to the Genome Engineering newsgroup (which...

  5. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...Dehydrogenase CUREs Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences...kinetics and mechanisms of action, regulation and post-translational modifications, and metabolic pathways...

  6. CRISPR References and Information

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who have deposited...Introductory video from the Feng Zhang lab Addgene Blog Posts How to Design Your gRNA for CRISPR Genome Editing...experiments. For more information, see the Addgene blog post from the Chan Zuckerberg Biohub . gRNA Design Deep...

  7. Huang Lab CRISPR Plasmids

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A human codon-optimized Cas9 nuclease expression...

  8. Calarco Lab CRISPR Plasmids

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to...

  11. Antibodies

    Type
    Collection
    ...choose, store, or use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody...

  12. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic modifications, researchers...

  13. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Assorted Cas9 nucleases from different bacteria ...

  17. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems, including the enzymes Cas13a...

  18. Viral Production

    Type
    Collection
    ... function. These data are sometimes reported or posted on the material page for the corresponding catalog... of early passage cells. Approximately 2-3 weeks post-thaw, cell culture supernatant is tested for mycoplasma...
Showing: 1 - 20 of 756 results