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...methods. Read more in our Restriction Cloning blog post . Figure 1: Restriction enzyme cloning of your gene...conditions. Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created ...enzyme cloning. Read more in our TOPO Cloning blog post . Figure 3: Summary of TOPO cloning. TOPO = topoisomerase...exonuclease. Read more in our Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created ...project. Read more in our Golden Gate Cloning blog post . Figure 5: Summary of Golden Gate cloning. Created...secondary structures. Read more in our SLIC blog post . Figure 6: Summary of LIC. Created with BioRender.com...

...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

... and right homology arms. See Addgene's HDR blog post for more details. In some cases, there may be no... aureus Cas9. For more information, see our blog post on overcoming the AAV size limitation . Browse Addgene's...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods...expression? For more information, check out our blog post on validating CRISPR modifications . Cell Population...

...the goal is to identify those cells that survive post-selection. The selective pressure must be strong...our Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...outline for using lentiviral gRNA libraries in postivie or negative screens. The use of appropriate controls...

...below and recommend checking out Addgene’s blog posts about common E. coli lab strains and E. coli strains...resistance and additional antibiotics, see our blog post on antibiotic resistance genes . Below you will ...standards of our plasmid collection, see our blog post on QC sequencing technologies at Addgene . Sanger...

...Educational Resources From eBooks to videos and blog posts to protocols, our educational resources cover a ...

... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

...screens, see our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries...

...tissue-specific Cre is provided. Download our chemogenetics poster: References and Further Reading Atasoy, D., & Sternson...