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1. Adeno-associated virus (AAV) Guide

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   Guide

   ...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

2. Plan Your Experiment

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   Guide

   ... and right homology arms. See Addgene's HDR blog post for more details. In some cases, there may be no... aureus Cas9. For more information, see our blog post on overcoming the AAV size limitation . Browse Addgene's...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods...expression? For more information, check out our blog post on validating CRISPR modifications . Cell Population...

3. Guide to Using Pooled Libraries

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   Guide

   ...the goal is to identify those cells that survive post-selection. The selective pressure must be strong...our Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...

4. Educational Resources

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   ...Educational Resources From eBooks to videos and blog posts to protocols, our educational resources cover a ...

5. Molecular Biology Reference

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   Guide

   ...and recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli...listed in the table below can be found in this blog post . Below you will find a few antibiotics commonly...

6. Cloning

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   Guide

   ...information and tips check out our Plasmid 101 blog post on TOPO cloning. Back to Top Gibson Assembly (Isothermal...

7. Gamma-Retroviral Vector Guide

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   Guide

   ... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

8. Lentiviral Vector Guide

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   Guide

   ... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

9. CRISPR Guide

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   Guide

   ...screens, see our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries...

10. Chemogenetics Guide

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    Guide

    ...: FLEx Vectors blog! Download our chemogenetics poster: References Alexander GM, Rogan SC, Abbas AI, Armbruster...