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We narrowed to 393 results for: prep

Showing: 181 - 200 of 393 results

  2. Lentiviral Vector Guide

    Type
    Guide
    ...Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral Vectors 101 eBook Addgene's...Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are...

  3. Colony Formation Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...conical tubes, VWR 21008-216 Lentivirus preparation Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM...This protocol can be used to titer lentiviral preparations that confer antibiotic resistance. Protocols...This protocol can be used to titer lentivirus preparations that confer antibiotic resistance. Determining...Determining the titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI... Use this dose for the colony formation assay. Prepare a batch of DMEM complete containing 10 μg/mL polybrene...thaw the lentiviral aliquot on ice prior to use. Prepare 10-fold serial dilutions of the lentivirus into...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Packaging Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids ...more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution has ...were discovered as a contaminant of adenovirus preparations, which is how they got their name, but they ...

  5. DNA Quantification

    Type
    Protocol
    ...NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click ...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...15 mL conical tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin...puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-L-glutamine (...determine the lowest dose that kills all of the cells. Prepare a batch of DMEM complete + 10 µg/mL polybrene by...Thaw the lentiviral aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM ...cell solution in this step. To seed the cells: Prepare a batch of cells as follows: Dilute 350,000 cells...growing well and have been sufficiently expanded, prepare cell stocks and/or harvest to test for protein ...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...you can find a protocol for kit-free plasmid mini-prep at the bottom of this page. Last Update: Feb. 8,...culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow (low copy... Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions: Solution I - Resuspension...pellet the bacteria before proceeding with DNA preparation. Pro-Tip If your entire overnight culture cannot...

  8. Antibody Guide

    Type
    Collection
    ...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...

  9. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...appropriate imaging system. Figure 1 Recombinant antibody preps should have 2 clear bands at ~50 kDa and ~25 kDa... Pro-Tip Addgene only uses recombinant antibody preps that have a purity of ≥90% but some labs use unpurified...prestained protein ladder on ice. Reagent Preparation Prepare IgG Standard Dilute the 2.5 mg/mL IgG isotype.... Add 8 µL of PBS to a total volume of 13 µL . Prepare the recombinant antibody Dilute or concentrate ...microfuge tube. Add 8 µL of PBS to total 13 µL . Prepare 1X MOPS buffer Dilute 25 mL of 20X MOPS buffer ...

  10. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s... number of genome-containing particles of an AAV prep using SYBR green technology. This protocol was validated...each step Reagent Preparation Master Mix: Count the number of samples (n) and prepare master mix for an...μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2 x 10 9 molecules/μL to generate...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share ...alongside a journal article, a preprint, or without any paper at all. Posting preprints and openly sharing materials...reproducibility, and accelerate science. We encourage preprint authors to deposit materials without a hold or...or to release the hold as soon as the preprint is posted in order to support these goals. And we can update...article version any time later. We recognize that a preprint isn’t necessarily a clear sign that you are ready...your plasmids, so we won’t immediately release preprint-associated materials the way we will with journal...to consider sharing them openly as soon as that preprint is online!   Thanks for reading, and for depositing...

  13. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...confirm the identity and serotype of our AAV preparations. We recently published a description of the ... For labs that produce a small number of AAV preparations this level of quality control is sufficient ...facilities producing a large number of viral preparations in parallel, there is a very real risk of mixing...AAVs and the TA-based ligation and tagmentation preparation approaches described previously require a preliminary...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...simplified approach, we sequenced over 100 AAV vector preparations and showed that VGS can quickly and reliably... script that can confirm the serotype of AAV preparations.  Using viral genome sequencing to determine...

  14. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...first scientific poster, but unsure how best to prepare. Maybe you’re a presentation veteran, but have ...experience. No matter the reason for attending, your preparation and day-of game plan will ensure you have the...experience possible. Tips for poster session presenters Prepare for success You’ve put a great deal of thought ...it’s time to expertly show off your science.  Prepare the night before: To avoid rushing out the door...poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our posters... part of a poster session, but with a little preparation and a few deep breaths the day-of, you’ll handle...Strategically traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve...

  15. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...reference: OpenWetWare Preparing chemically competent cells (protocol)  Preparing electrocompetent cells... Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...Research to make competent cells because cells prepared using this kit can be transformed without heat...of electrocompetent cells (protocol)  Cells prepared using the Z-competent TM buffer can be easily ...

  16. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ...for poor quality slides with sufficient time to prepare (and it does take time). For important talks, ask...loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few extra slides...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...their phones. Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...consider using a different antibody.  Prepare a positive control Prepare and label a sample that expresses...paraformaldehyde-fixed cell samples, or other preparations. Sequential IF For a sequential IF experiment... ICC validated antibodies from Addgene), or be prepared to run some extra controls and validate the antibody...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...

  18. Sequencing Primers

    Type
    Guide
    ...Qiagen) 5' of MCS in pQE vectors, forward primer pREP Forward GCTCGATACAATAAACGCC (Invitrogen) Rous sarcoma...

  19. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ... a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...containing instructions on how to prepare your plasmids and a prepaid label with shipping materials to ...journal. To start this process, simply enter your preprint title or a general description of the deposit....

  20. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ... can prepare you to present your work at conferences, help with manuscript and grant preparation, and ...science communication skills. Ultimately, my degree prepared me to be exactly the type of scientist I wanted...interview with them to learn how their master's degree prepared them for their career.  2. Are you looking for...
Showing: 181 - 200 of 393 results