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We narrowed to 611 results for: sam

Showing: 181 - 200 of 611 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...seems obvious, it is important to remember that the same is true when designing gRNAs for using CRISPR technology...an alternative to HDR for introducing edits. The same locational constraints are even more exquisite for...concordance between different sequences targeting the same gene, suggesting that off-target effects did not...multiple gRNAs of different sequences give rise to the same phenotype in order to conclude that the phenotype...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...between samples. In addition to a DNA ladder standard, it is also a good idea to run an uncut sample of each...you to either stain your gel after you run your samples or add the stain as the gel is being made (post...containing ligation mixture with insert and backbone. Sample results indicative of successful and unsuccessful...

  3. Typing CRISPR Systems

    Type
    Blog Post
    ...data. In some cases, CRISPR systems that are in the same class and type don’t share sequence similarity but...between two types, the Cas effector may have the same name but be completely different! They are merely...but it’s suspected that they don’t carry out the same types of adaptive immune functions that other CRISPR... 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan...

  4. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that the red laser excites at...fluorophore-conjugated antibodies together in the same color panel. In this case, make sure to compare ...overlap. Hence, they should not be used within the same panel.     Figure 4: Excitation and emission...virtually overlap, you cannot use them together in the same panel. Source: https://www.bdbiosciences.com/en-...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...campus speakers thoughtfully reminded us to use the same critical lens on our scientific community as we ... nor would you mention my small error and at the same time pass over in silence his very large one” (http...exoplanets into a successful PhD thesis. We use the same atmospheric physics to understand exoplanets that...happy to see so many others come out who feel the same.  I was also incredibly impressed with how well ...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... However, the acceptor doesn’t need to go to the same exact location as the donor. As long as some of ...targeted nearby (such as a neighboring house on the same street as the donor), then FRET measurements are...Ebright, 2001). These Cy/NTA conjugates work the same way as NTA-sepharose beads used for protein purification... binding sites are adjacent to each other on the same protein and a negative control experiment conducted...

  7. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...the latest news and encourage your peers to do the same. Long-term lab conservation goals Lastly, I think...freezer to -70 °C and teach other labs to do the same. -70 °C is cold enough to keep some common types... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have... joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI) chaining results...photo-label multiple mitochondrial populations within the same cell. Mitochondrial fusion is typically assayed ...different mitochondrial subpopulations within the same cell; by photoswitching PSmOrange and photoactivating... and more, to your protein of interest using the same vector system. The tRNA/aaRS pairs are delivered... experiments in several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel ...

  9. RUBY-Red Siliques

    Type
    Blog Post
    ...GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has been ...consisting of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and...transformation next to an untransformed seed from the same plant beginning to germinate. (Top right, second...

  10. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...endogenous WT levels of the same protein when both are expressed in the same system. Tagging can also aid...EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative charge with 4 negatively...

  11. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...accommodate adeno-associated viral vector (AAV) samples and created a simplified process, termed viral ...parallel, there is a very real risk of mixing up samples, serotypes, or both. Unfortunately, the standard... as [+] and [-] strands that are packaged at the same frequency, we reasoned that, following DNA extraction...

  12. Plasmid Cloning by PCR

    Type
    Blog Post
    ...about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that...restriction enzymes that can both function in the same buffer, as this will save time later In our example...As such, the later steps in this process are the same as those discussed in our restriction cloning post...

  13. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    .... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

  15. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... DIY Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

  16. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...

  17. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic... a reference protein that is translated from the same open-reading frame and separated from the protein...brightness for a set of fluorescent proteins (with the same reference protein) under identical conditions, a...

  18. Antibodies 101: Isotypes

    Type
    Blog Post
    ...of antibodies, although not all isotypes have the same ability to activate each of these functions: Recruitment...appropriate isotype control. An isotype control has the same constant heavy chain domain as your primary antibody...results in the B cell expressing an antibody with the same variable domain but a different heavy-chain constant...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...opportunity to meet people who are interested in the same things you are on a deep level. Right away you have... and grow over time, but being interested in the same phosphate of your favorite kinase is a good start...meet someone since you are both interested in the same talk. Read more on this advice to “never leave an...
Showing: 181 - 200 of 611 results