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We narrowed to 779 results for: scr

Showing: 181 - 200 of 779 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine...overcoming the limitations listed above. First, they screened a variety of fluorescent proteins to find those... via immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the...addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to...

  2. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

  3. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Find the anti-CRISPR plasmids Tagging human transcription factors with GFP Kevin White’s lab recently ...deposited a set of CRISPR plasmids to tag human transcription factors with GFP. As part of the Encyclopedia...these donor plasmids will help researchers map transcription factor localization and binding via ChiP-seq...ChiP-seq. Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR...

  4. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...tools in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly...site to cleave and inactivate any read-through transcripts of the effector gene. Effector genes, any gene...across cell types and organisms by replacing the transcription factor binding site and effector in the construct... the SPARC system to use other experimental transcription activation systems, such as LexA/p65 or mCD8...

  5. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...CRISPR technology has brought drug and genetic screens together and expedited the drug discovery pipeline...Alternatively, a disease model can be generated, and drugs screened for a change in pathology. If the disease pathology...accelerate the drug pipeline. High-throughput CRISPR screens can be used to inhibit, activate, or knockout many...identified several candidate inhibitors of gene X in a screen. You generate a gene X knockout in a cancerous ...

  6. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...to present your work at conferences, help with manuscript and grant preparation, and lead your own research...

  7. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon on the right?!? ... course more quickly than writing scientific manuscripts and, as one of my supervisors warned me, the ...’t have to mean abandoning it or running away screaming, but instead can involve walking alongside it ...it and giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share ...

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more CRISPR and Genome Engineering...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...see: Uhlen et al., 2016). In this post, we will describe the approaches that can be used to determine selectivity...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...commercially available. Various proteomic and transcriptomic datasets can be used to select a candidate ...recognized by either antibody was not shared. Tables describe that the antibodies both recognized the target...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

  10. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    ...more detailed level, the Gal4/UAS system is a transcription activation system co-opted from yeast (1). It...workhorse to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences...sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus...Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703...

  11. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... lacks context unless it links to a verified description of that reagent online. Furthermore, if the link... link between the catalog number and reagent description is lost (e.g. a supplier is bought out by another...specific information about the plasmid: name, description, how the plasmid was generated, validated sequence...our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down to the bottom of this plasmid’s page you will...

  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...CRISPR pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular... infect target cells, and, finally, conduct the screen. To help alleviate some of this labor for our ...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...) says: "When you're setting up to do a genetic screen, you need to focus on building a really good model...

  13. Components of CRISPR/Cas9

    Type
    Blog Post
    ... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...arrays as found in the bacterial genomes are transcribed into pre-crRNAs containing both the spacer region...RNase III, the tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound...

  14. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...responses are varied; one outcome is directed transcription from a given promoter. To harness the power ...the RR (OmpR) via phosphorylation, promoting transcription (see figure below). Subsequent exposure to red...response regulator via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red...types of proteins, including Cas9 and dCas9-transcription factors, for which low expression levels are...

  15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different..., and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9, SpCas9-NG, which...Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to target multiple...post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed...

  16. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...recognized as important regulators of post-transcriptional gene expression across the majority of cellular...al., Nat Commun, 2019. PubMed PMID: 29934631. Screening immune cell lineages in vivo using the CRISPR-...tools have been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based...gene. CHIME can also be used for high throughput screens using pooled libraries. Find the CHIME plasmids...

  17. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...plasmid to express the target antigen could be screened in a high-throughput manner. Cells were labeled...which was observed for the hybridoma mAb.  While screening by IF-ICC worked well for identification of the...was recognized that an aberrant IgG light chain transcript was expressed by some of the hybridomas and, ...limited the number of clones that needed to be screened through functional assays. Following functional...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...catalog – so check there before building one from scratch! Why deliver these systems virally instead of by... preserving cell viability. Library delivery Screening libraries, large collections of biological independent... entire genome, overexpression of all human transcription factors, barcoded libraries to trace cell lineage...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...

  20. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila...Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists to study...red. The variation in color depends on how transcriptionally active the genomic locus of integration is...Using a Drosophila cell line, I’ve studied a transcription factor important for T cell differentiation ...
Showing: 181 - 200 of 779 results