We narrowed to 254 results for: sms

1. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...

2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...their presence. They are genetically modified organisms, and GMOs have a bad reputation. The public is...

3. Truncated gRNAs for Regulating Gene Expression

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   ...double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to either inactivate a ...

4. 10 Basic tips for mammalian cell culture

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   ...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...

5. Common Injection Routes in Mice

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   ...Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering...

6. Production of Virus in Insect Versus Mammalian Cells

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   ...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...

7. Viral Vectors 101: Voltage Indicators

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   ...vectors become invaluable. Viral vectors are mechanisms by which genetic components can be delivered ...

8. Plasmids 101: Degron Tags

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   ...CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced...

9. Of Myc and Men

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   ...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...

10. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working with FPs...

11. Addgene's Cancer Collection Pages Connect the Research Community

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    ...cancerous cells Luckily, there are several safety mechanisms in place to protect us from rogue cells when ...

12. Google Forums Round Up: First Impressions of NgAgo

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    ... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...

13. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...

14. Light Sheet Fluorescence Microscopy

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    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...

16. MXS Chaining

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    ...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The...

17. Uncovering Ancestral Bias in CRISPR Research

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    ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

18. Antibodies 101: Producing Recombinant Antibodies

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    ...25819219. Wang W, Roberts CJ. Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct...

19. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...

20. Viral Vectors 101: The Retroviral Lifecycle

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    ...genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...