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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 991 - 1000 of 1010 results

  1. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...with the razor blade. This is especially important during the DNA purification step, as many kits cannot ...used to determine how much of each buffer to add during the DNA isolation step. Finally, you will want ...

  2. Ligation Independent Cloning

    Type
    Protocol
    ... cloning methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp)...annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for LIC typically...

  3. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...Guidelines BSL-1 Guidelines Before You Work Right after entering the lab, put on the proper personal protective...concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste can be collected...

  4. Immunocytochemistry

    Type
    Protocol
    ...cells, target proteins, etc. Sharing speeds science. We believe that sharing the full details of our protocols...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...immediately or stored at -20 °C until ready to use. Preparing for ddPCR Thaw samples, primers/probe mixes, and...samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the risk of contaminating reagents...

  7. Protocol - How to Design Primers

    Type
    Protocol
    ...inosine) instead of a regular nucleotide for broader pairing capabilities. Taking into consideration the information...
Showing: 991 - 1000 of 1010 results