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Showing: 101 - 110 of 341 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...proprietary. These methods also rely on ssDNA ends to match up regions of homology, so they won’t work if the...L-arabinose. These cells can be made and frozen in large batches suitable for many experiments. When REPLACR-mutagenesis...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...quite variable from antibody to antibody or batch to batch. For example, take lysine conjugation, which...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... important step in ensuring quality, as it will catch any errors made during the cloning, sample handling... sequences to visually check for homology and mismatches. Do you have your own best sequencing practices...

  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication or as ...spreadsheet, save it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...standard conditions be used when comparing different batches of virus. In addition, some viral preps are very... stored in the freezer. If you plan to freeze a batch of virus for long term use, you may want to consider...

  9. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...calculations so that your assay output number (RQ value) matches the copy number of your sample. In that case, an...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...additionally remove all jewellery as well as that watch you’re wearing before you start. Also, avoid unnecessary...cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods should not ...
Showing: 101 - 110 of 341 results