New Optogenetic Tools for Cytoskeleton and Membrane Control
Type
Blog Post
...limitations (Figure 1).
Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules:
VVDfast homodimerizes under blue light
iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...