...the producer cells and purification protocols often describe a process that begins with cell lysis to release... steps to separate cell culture supernatant containing exo-AVVs from producer cells, and then to enrich...effectively target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma...particles is able to infect more cells (as demonstrated in different cellular environments such as retina ...Exosomal vesicles secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic...Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production... to be spontaneously released from the producer cells into the supernatant during the production phase...

...improved cell-free system to examine biosynthetic pathways A new tool for enriching ABE-targeted cells The ...interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free system,...successfully reconstituted in this cell-free system. In summary, this cell free system allows for a relatively...multiplexed and can be used in human pluripotent stem cells, a cell type that has been difficult to modify in the... Corner New ready-to-use viral preps   Improved cell-free transcription/translation system to examine...biosynthetic pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred....0c00581   New tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through...

...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing...viral entry into the host cell.  Transfecting these three plasmids into cells enables the production of...lost through cycles of cell division, though the rate of loss greatly depends on cell turnover. Why use ...be different for each transduced cell, there’s no telling which cells may harbor these unwanted interruptions...expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells, as they avoid the... chromosome of the cell. This integrated viral genome is then part of the cellular DNA… forever!  This... transduced cells and quantifying the integrated viral DNA along with a reference cellular gene. When ...

...primary human cells  Cell therapy approaches can use base editors to correct mutations in stem cells and return...primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently... In addition to stem cells the authors targeted 6 genes in primary human T cells using 8 selected ABE8s...to edit primary human T cells efficiently makes it a promising tool for T cell therapy development.  Reducing...be introduced into cells as a plasmid or as mRNA constructs. In primary human cells mRNA delivery of ABEs...ability for more robust editing in human cell lines and primary cells makes them a great addition to the base...these base changes, especially in primary human cells. Now, scientists at Beam Therapeutics have found...

...cancerous cells Luckily, there are several safety mechanisms in place to protect us from rogue cells when ...process of programmed cell death, or apoptosis, is one such mechanism. When a cell or its genetic material... directly inhibit the ability of a cell to undergo programmed cell death while simultaneously stimulating...eliminate every cancerous cell. Even so, to pose the greatest threat, cancerous cells still need to successfully...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division. This...proteins that, when expressed, normally encourage cell survival, growth and proliferation, while tumor ... suppressors perform the opposite task, keeping cells from dividing out of control. One may compare the...

...with live host cells Now you can proceed with incubating this membrane with your cells of interest and...membranes well with PBST to remove unbound cells. To detect bound cells, simply lay each membrane on an agar... cloned into E. coli cells, isolated colonies will be lifted onto nitrocellulose membranes and exposed... proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity.  First, cut...E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...directly onto nitrocellulose and carry out the steps from blocking the membrane to probing with cells in order...structure(s) a given phage will recognize on a host cell. To make matters even more interesting, much of...

...demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given the long ... expressed in a monolayer of endothelial cells, showing cell protrusions and overlaps (arrows) after blue...2016). Rho GTPase signalling in cell migration. Current Opinion in Cell Biology, 36, 103–112. https://doi.org...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...optogenetic tools to control the cytoskeleton and cell membrane dynamics. First, a quick refresher.  The...important components of the cytoskeleton. They provide cells with physical structure, separate chromosomes during...strategies are available to disrupt microtubules in cells, they have various disadvantages and limitations...

... pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with a Genetically...SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):...biosensor for visualization of acetyl-CoA in live cells. Cell Chem Biol. 2025 Jan 21: S2451-9456(25)00002-9... Tantama ATP QUEEN ATP sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative...for Imaging Insulin Secretion in Single beta Cells. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. Dmytro...accessibility. Cell. 2022 Sep 1;185(18):3390-3407.e18. David Clapham Return to Top Cell Physiology Target...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...

... mammalian cells 48880 3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD...mammalian cells 49536 3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9...Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. Fujita T, Kitaura F...enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-IG Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-I2 Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...

...at bioRxiv. AAV2 interacts with the packaging cell's cell membrane When packaging adeno-associated viral...of the packaging cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant... particles associate with the cell membrane and are taken into the cell via endocytosis. In our study,...AAV2 viral particles by our packaging cells by treating the cells with the endocytosis inhibitor sucrose...target cells is blocked following sucrose treatment. Moreover, several reports have shown that cells treated... yields approximately 1.8-fold without affecting cell viability. This effect appears to be serotype specific...difference between untreated and sucrose treated cells. Although we were unable to deduce the mechanism...