We narrowed to 144 results for: transfection

...too young and should not be used for important transfection experiments right after they’ve been thawed....

... adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and...

...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem...

...loci within a single cell. One might imagine transfecting cells with both the nuclease-positive and nuclease-null...

...tag. The two PCR reactions are then mixed and transfected along with a Cas9 expression vector. They can...

...Day 2: b. Perform the transfection in the late afternoon because the transfection mix should only be incubated...pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles...transient knockdown of protein expression, you may transfect plasmid DNA directly into the target cells. The...#12259 HEK-293T cells GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001...Producing Lentiviral Particles This protocol is for transfection in a 6 cm plate. The protocol can be scaled ... as needed. Day 1: a. For each plasmid to be transfected, plate 7×105 HEK-293T cells in 5 mL of media ...polystyrene tubes), make a cocktail for each transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging...

...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...