We narrowed to 658 results for: CHI;

...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...

... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...

... Delivery of AAV into the brain can usually be achieved by a directed brain injection, but some serotypes...types. Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory...

...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in...

...been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

...fluorescent proteins are, which makes mixing and matching a relatively easy proposition, explained Michelle...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...

...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

...biomedical researchers and ready access to stably archived large-scale datasets by computational biologists...interest using a simple keyword search. For example, searching the term "breast cancer" returns 40 screens performed...

...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

...been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re proud to...scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed the way in which...

...fifth graders, made a blog and worked as editor-in-chief for the Science in the News Blog, a blog made by...researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking, time management...

...Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease specificity...even compared his "memory" gene codes to a Turing machine (also discussed in the book). These synthetic biologists...

... Flexibility to manage their personal lives to achieve a healthy work-life balance. It is harder to manage...responsibilities, which might include training or teaching others, research, and policy development. Every...

...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

...PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important factors to consider...months reading and writing a proposal before even touching a pipetteman. Take the time to generate new ideas...

...sgRNAs, but they may or may not include CRISPR machinery (which can be supplied separately). Libraries ...systematically explore variations in the spike protein, teaching us more about viral evolution and antibody binding...

...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

...Glick from SnapGene!  Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...

...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ...

...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

...accompany him to YNP the following year. Geoff Schiebinger, another Quake Lab member joined for half of ...in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due to the 3,471 square...

...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...