We narrowed to 650 results for: CHI;

1. Tag Your Favorite Yeast Genes with Ease

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   ... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...

2. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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   ... Delivery of AAV into the brain can usually be achieved by a directed brain injection, but some serotypes...types. Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory...

3. pSiM24: Simplifying Plant Genetic Engineering

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   ...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in...

4. Mapping the 4D nucleome with CRISPR/Cas9

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   ...been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

5. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   ...fluorescent proteins are, which makes mixing and matching a relatively easy proposition, explained Michelle...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

6. In Living Color: The Skinny on In Vivo Imaging Tools

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   ...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...

7. Plasmids 101: What is a plasmid?

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   ...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

8. Sensing Neuronal Dopamine

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   ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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   ...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

10. SunTag and Fluorescent Imaging

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    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

11. Bricking Science: Portraying Scientific Reality Through LEGO

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    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

12. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

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    ...been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re proud to...scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed the way in which...

13. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    ...biomedical researchers and ready access to stably archived large-scale datasets by computational biologists...interest using a simple keyword search. For example, searching the term "breast cancer" returns 40 screens performed...

14. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ...fifth graders, made a blog and worked as editor-in-chief for the Science in the News Blog, a blog made by...researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking, time management...

15. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    ...Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease specificity...even compared his "memory" gene codes to a Turing machine (also discussed in the book). These synthetic biologists...

16. Management for Scientists: What Makes a Good Manager Anyway?

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    ... Flexibility to manage their personal lives to achieve a healthy work-life balance. It is harder to manage...responsibilities, which might include training or teaching others, research, and policy development. Every...

17. Choosing Your Perfect Empty Backbone

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    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

18. Advice for Choosing a Research Project

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    ...PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important factors to consider...months reading and writing a proposal before even touching a pipetteman. Take the time to generate new ideas...

19. Hot Plasmids and Viral Preps - September 2021

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    ... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ...

20. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

21. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

22. New and Upcoming Viral Vectors - Spring 2019

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    ...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

23. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

24. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

25. Science Careers: Unruly Interests Feed Many Paths

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    ...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...

26. Identifying Sequence Elements with SnapGene's Feature Database

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    ...Glick from SnapGene!  Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

27. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...accompany him to YNP the following year. Geoff Schiebinger, another Quake Lab member joined for half of ...in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due to the 3,471 square...

28. Addgene's Top 10 Blog Posts of 2015

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    ... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ...

29. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent...

30. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...