We narrowed to 877 results for: HA

...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ...

...last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing...that regulate a desired phenotype. In fact, CRISPR has already been used to engineer the genome of many ...to protoplast cells (plant cells whose cell wall has been removed). Another common delivery method is ...developed for plants and prime editing for plants has been developed for rice by Yiping Qi’s lab and for...

...sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey has been a tough ...

...Hack Day lunches These days everyone is busy and has a To-Do list long enough that there is no hope of...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams. ...

...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the...always look at your antibody datasheet to see if it has been tested in IF and if a protocol is available....an effective strategy. (This article on fixation has some more examples to help choose which fixative ...

...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting ...Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible...that is smaller and easier to deliver, but so far has few inhibitors identified.     New from the viral...

... of practice to ensure the proper injection site has been located and to avoid potential off-target pokes...researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing...: intermediate Your decision Hopefully this post has provided a good introduction to common injection ...

...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...biosensors, and generally as biological tools, our blog has you covered. Happy voltage sensing!   Figure...

...methods for CRISPR activation, research in the area has continued to develop. These newer methods have not... of rearranging chromatin structure. This method has shown higher activation levels than SAM in some loci...-HSF activation domains used in SAM. This method has been shown to activate genes at 2- to 3-fold higher...

...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...the bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition...

...DRSC/TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down...tools. The Vienna Drosophila Resource Center also has collections of flies for in vivo gene knock down ...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...looked hard at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in the Biohub....

...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of the sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have...the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ’s dominance ...

...expensive, it may be helpful to find an antibody that has already been validated for flow cytometry through...Characterization through Open Science) initiative has now started producing flow cytometry characterization...overexpression. Additionally, if the cell line used has endogenous levels of expression, this can make the...TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop...as part of the YCharOS open science ecosystem. It has received in-kind support to produce this data from...

...buffer such as TE. You will now have plasmid DNA that has been purified away from the bacterial proteins and...

...cooperative education experience (co-op) is what has brought me, a second year bioengineering student,... that remote work would feel isolating, but that has not been the case for me at Addgene. There is a clear...

...bacteria in real time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome... can colonize the mammalian intestinal tract and has been used for 100 years to clinically treat enteric...

...research and PhD studies, there lies a hidden gem that has the potential to redefine the course of one's research...inspiration. Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint...

...about whether Natronobacterium gregoryi Argonaute has any DNA editing functionality Collecting preclinical...extension also tells readers if a paper they’re reading has been linked in a ReFigure making it easy for others...

...manipulated, its anatomical origin, if cell line has ever been the target of an ‘-omics’ study or is resistant...can be used in some cases, but much of this work has required manually going through the pdfs of the 26,000...

...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for ...mosaic virus, the constitutively active M24 promoter has been modified with duplicated enhancer domains and...

...keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our...the same time, he pointed out, academic research has some big problems resulting from past practices and...

...form only weak and reversible bonds. Howarth’s team has already demonstrated in their PNAS paper that SpyLigase...discovered the domain. It is small, highly stable and has this amazing chemistry locking the domain together...

...research project can take several years to complete and has multiple benchmarks. Think about what your personal...Have a look at what others have done recently that has been a big hit, and especially look at the new tools...

...overexpression. Tim Formosa, at the University of Utah, has built a complete collection of yeast tagging modules...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site...

...Toronto KnockOut (TKO) library from the Moffat lab has over 175,000 different gRNA-containing plasmids)....

...agents. Directly conjugating the primary antibody has the additional benefit of permanent linkage. This...steps following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak... the scope of this post.  We hope that this blog has provided you with a useful overview of the IP method...

...fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection...putative T1 transformants: When your T1 is on soil and has recovered from the stress of selection you can clip...cell fate maintenance in the stomatal lineage and has made countless transgenic plants in the process. ...

...Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...often under-appreciated or over-looked.  Synthego has developed a new tool called ICE (Inference of CRISPR...increased flexibility and scalability, the ICE software has two analysis formats: sample by sample analysis, ...

... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...  Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your...refers to the number of cysteine sites the nanobody has available for conjugation to a specific label (see...

... codon table — a chart every molecular biologist has seen before, showing how DNA sequences are translated...lost from the population. A bacterial chromosome has a single origin of replication (ori), where DNA replication... you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even in cells with ...

...studying mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult...editing (PE), initially developed in human cells, has been uniquely challenging to apply in dicotyledonous...

...depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb...., or reagents and equipment. Hopefully this post has provided some insight on these differences in viral...

...et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions ...yet to be demonstrated (or invented), its behavior has already been successfully reproduced by several other...tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...

...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

...months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated...contains requests from 5/22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests...

... following IV injection. Compared to AAV9, PHP.B has a >40-fold increase in CNS transduction and transduces...adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 gene... line of viral vector permissiveness While PHP.B has impressive CNS transduction in some strains of mice...

... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...using the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that ...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...