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We narrowed to 849 results for: HA

Showing: 401 - 440 of 849 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...overexpression. Tim Formosa, at the University of Utah, has built a complete collection of yeast tagging modules...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site...

  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

  4. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...agents. Directly conjugating the primary antibody has the additional benefit of permanent linkage. This...steps following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak... the scope of this post.  We hope that this blog has provided you with a useful overview of the IP method...

  6. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...often under-appreciated or over-looked.  Synthego has developed a new tool called ICE (Inference of CRISPR...increased flexibility and scalability, the ICE software has two analysis formats: sample by sample analysis, ...

  7. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...  Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your...refers to the number of cysteine sites the nanobody has available for conjugation to a specific label (see...

  8. Viral Production at Addgene

    Type
    Blog Post
    ...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... codon table — a chart every molecular biologist has seen before, showing how DNA sequences are translated...lost from the population. A bacterial chromosome has a single origin of replication (ori), where DNA replication... you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even in cells with ...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...studying mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult...editing (PE), initially developed in human cells, has been uniquely challenging to apply in dicotyledonous...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb...., or reagents and equipment. Hopefully this post has provided some insight on these differences in viral...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions ...yet to be demonstrated (or invented), its behavior has already been successfully reproduced by several other...tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

  14. MXS Chaining

    Type
    Blog Post
    ... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...using the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that ...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ...fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection...putative T1 transformants: When your T1 is on soil and has recovered from the stress of selection you can clip...cell fate maintenance in the stomatal lineage and has made countless transgenic plants in the process. ...

  16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...properties of modified histones, a genomic region that has mono-methylated and acetylated histones, but not ...magic and Poisson statistics, each sub-population has, on average, one distinct enhancer disrupted. As ...unhealthy cells. Until recently, the human genome has been full of dark matter: enhancers, lncRNAs, repetitive...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...the State Museum has been the best supporter and most productive partnership CINSO has had. Volunteer! ...provide online. Twitter is an excellent resource that has gained a lot of traction with researchers. It is ...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...but the introduction of type V Cpf1-driven systems has added another option to the CRISPR toolbox. Cpf1’...frequency of desired HDR edits, but the possibility has not yet been experimentally confirmed. To prevent...adaptation of Cas13 to RNA targeting and RNA editing, has further shown the diversity of CRISPR-based systems...

  20. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...plasmid we have at Addgene. Each sample at Addgene has four layers of location which can all be extrapolated...hold up to 96 tubes (see image below). Each plate has numbers and letters to give coordinates, from A1 ...replicate sample. The transformation After the DNA has been received and stored, it is put into E. coli ...

  21. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...cleavage with some of these peptides, no consensus has been reached on which peptide works best. Likely ... is easiest to start with a plasmid that already has the multicistronic element and reporter cloned in... unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic...

  22. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... Lab technician / Research assistant Typically has a Master's or Bachelor's degree Generally a short-term...talking to people, though, try to be conscious of who has your best interests at heart. A great piece of advice...vulnerable about my struggles and what I don’t know yet has helped me cultivate stronger relationships with my...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career options...

  23. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...extraordinary collection of talent, of human knowledge, that has ever been gathered at the White House - with the ... not an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  ... on a leaderless team. Sometimes the leader just has to be the person to say “OK, guys, we need to have...anything out the discussion themselves, then one has to question whether that person should be on the ...

  24. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... technique developed in David Liu’s lab in 2019, has added considerably more precision and flexibility... the canonical base editing window, base editing has higher efficiency and fewer indels than prime editing... repair (HDR) stimulated by double strand breaks has been widely used to generate precise changes. However...nicking of the unedited strand only after editing has occurred and reduces indels by 13-fold (Anzalone ... al., 2024; Yarnall et al., 2023) Prime editing has a lot of promise for treating genetic diseases. In...

  25. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...techniques and troubleshooting tips. The fourth edition has been fully updated with twelve new sections, including...

  29. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

  30. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg. Plasmid Serotype Name...

  31. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... power of science to improve the world around us has long been known; ensuring all have access to this...information becomes easier to access by the day, it has never been more crucial to understand just how we...

  32. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...the field and terminology that is widely used but has a very specific use-case (definition) in a specific...specific field.  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the ...

  33. RUBY-Red Siliques

    Type
    Blog Post
    ...large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright red pigment... University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful imaging.   ...

  34. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...available, the next best thing is a protein that has been validated for an assay that uses similar sample...Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western because...

  35. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...on single-cell gene expression patterns. The lab has also used CellTags for tagging two cell populations...libraries at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...

  39. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

  40. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...
Showing: 401 - 440 of 849 results